A mussel-derived one component adhesive coacervate.
A mussel-derived one component adhesive coacervate.
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DOI：
10.1016/j.actbio.2013.09.007
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发表时间：
2014-04
影响因子：
9.7
通讯作者：
Waite, J. Herbert
中科院分区：
文献类型：
--
作者：
Wei, Wei;Tan, Yerpeng;Rodriguez, Nadine R. Martinez;Yu, Jing;Israelachvili, Jacob N.;Waite, J. Herbert
关键词：
海洋生物以复杂流体的形式处理和提供许多水下涂料和粘合剂。在海洋贻贝中，一种这样的流体，在粘附斑块形成期间分泌，由称为Mfp-3S的贻贝足蛋白（mfp）的浓缩胶体悬浮液组成。这项研究的结果表明，Mfp-3S成为一个复杂的流体，从平衡溶液中的pH值和离子强度的液-液相分离，让人想起的条件下创建的贻贝脚在菌斑形成。相分离的pH依赖性及其敏感性表明，分子间/分子内静电相互作用是部分负责驱动相分离。非极性Mfp-3S蛋白之间的疏水相互作用为凝聚提供了另一个重要的驱动力。由于复合凝聚通常由聚阴离子和聚阳离子之间的电荷-电荷相互作用产生，因此Mfp-3S是唯一已知的与自身凝聚的蛋白质。Mfp-3S凝聚层显示具有≤ 1 mJ/m2的有效界面能，这解释了其在大多数表面上扩散或吞没的趋势。对生物医学应用特别感兴趣的是凝聚的Mfp-3S在羟基磷灰石上的极高吸附能力。
Marine organisms process and deliver many of their underwater coatings and adhesives as complex fluids. In marine mussels, one such fluid, secreted during the formation of adhesive plaques, consists of a concentrated colloidal suspension of a mussel foot protein (mfp) known as Mfp-3S. Results of this study suggest that Mfp-3S becomes a complex fluid by a liquid-liquid phase separation from equilibrium solution at a pH and ionic strength reminiscent of conditions created by the mussel foot during plaque formation. The pH dependence of phase separation and its sensitivity indicate that inter/intra-molecular electrostatic interactions are partially responsible for driving the phase separation. Hydrophobic interactions between the nonpolar Mfp-3S proteins provide another important driving force for coacervation. As complex coacervation typically results from charge-charge interactions between polyanions and polycations, Mfp-3S is thus unique in being the only known protein that coacervates with itself. The Mfp-3S coacervate was shown to have an effective interfacial energy of ≤ 1 mJ/m2 which explains its tendency to spread over or engulf most surfaces. Of particular interest to biomedical applications is the extremely high adsorption capacity of coacervated Mfp-3S on hydroxyapatite.
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影响因子：
10.7
作者：
de Kruif, CG;Tuinier, R
通讯作者：
Tuinier, R
影响因子：
5.5
作者：
Kausik, Ravinath;Srivastava, Aasheesh;Han, Songi
通讯作者：
Han, Songi
影响因子：
3.4
作者：
Spruijt, Evan;Sprakel, Joris;van der Gucht, Jasper
通讯作者：
van der Gucht, Jasper
影响因子：
3.3
作者：
Ganzevles, Renate A.;Kosters, Hans;de Jongh, Harmen H. J.
通讯作者：
de Jongh, Harmen H. J.
DOI：
10.1111/j.1432-1033.1997.00092.x
发表时间：
1997-11-15
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Vrhovski, B;Jensen, S;Weiss, AS
通讯作者：
Weiss, AS