Electrically induced neurite outgrowth of PC12 cells on the electrode surface
Electrically induced neurite outgrowth of PC12 cells on the electrode surface
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DOI：
10.1007/bf02523221
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发表时间：
1998-07-01
影响因子：
3.2
通讯作者：
Aizawa, M
中科院分区：
文献类型：
--
作者：
Kimura, K;Yanagida, Y;Aizawa, M
在缺乏神经生长因子（NGF）的情况下，电刺激诱导PC12细胞在氧化铟锡（ITO）电极上的形态分化生长出神经突。在电极上施加矩形脉冲波电位，振幅为200 mV和400 mV，频率为50 Hz， 500 Hz和1 kHz。PC12细胞在200 mV和100 Hz下分化最明显。电诱导的神经突长度之间无统计学差异。用LaCl3阻断钙内流可完全抑制电诱导分化。这表明在细胞膜附近反复的电位转移可能通过钙离子通道刺激形态学反应。
Morphological differentiation of PC12 cells cultured on an indium-tin oxide (ITO) electrode has been induced to grow neurites in the absence of nerve growth factor (NGF) by electrical stimulation. Rectangular pulse wave potentials were applied to the electrode at amplitudes of 200 mV and 400 mV with frequencies of 50 Hz, 500 Hz, and 1 kHz. The PC12 cells differentiated most prominently at 200 mV with 100 Hz. No statistically significant differences were observed among the electrically induced neurite lengths. The electrically induced differentiation was completely inhibited by a blockade of calcium influx using LaCl3. This indicates that repeated potential shift in the vicinity of a cellular membrane may stimulate morphological response, probably through calcium ion channels.