MALDI-TOF "fingerprint" phospholipid mass spectra allow the differentiation between ruminantia and feloideae spermatozoa
MALDI-TOF "fingerprint" phospholipid mass spectra allow the differentiation between ruminantia and feloideae spermatozoa
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DOI：
10.1016/j.theriogenology.2008.08.023
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发表时间：
2009-03-01
期刊：
影响因子：
2.8
通讯作者：
Mueller, K.
中科院分区：
文献类型：
--
作者：
Fuchs, B.;Jakop, U.;Mueller, K.
对精子脂质的详细比较分析将有助于更好地理解膜在受精过程中的功能，以及精子的保存。敏感的分析方法的应用是特别必要的濒危物种作为精子的可用量（和，因此。可提取的脂质）受到强烈限制。基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱（MS）是一种快速、简便、灵敏的方法，可用于测定几种反刍动物（牛、梅花鹿、Klipspringer）和猫科动物（家猫、东北虎、fosa）精子的磷脂组成。特征“指纹”是从正离子光谱中获得的，可以区分两个动物组。反刍动物精子的脂质提取物主要含有醚脂质，包括必需量的缩醛磷脂，与此相反，猫亚科精子的更复杂的磷脂组成明显由二酰基磷脂主导，仅含有少量的缩醛磷脂。同时也表明，同一物种的射精精子、电射精精子和附睾尾精子的脂质组成非常相似，并给出了可比较的数据。因此，对射出精子的分析不是绝对必须的。(C)2009年由Elsevier Inc.出版
The detailed comparative analysis of sperm lipids could essentially contribute to abetter understanding of membrane function in the context of fertilization and, moreover, of sperm preservation. The application of sensitive analytical methods is particularly necessary for endangered species as the available amount of spermatozoa (and, accordingly. extractable lipids) is strongly limited. It will be shown that matrix-assisted laser desorption and ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) is a fast, simple and sensitive method for the determination of the phospholipid composition of spermatozoa from several ruminantia (cattle, roe deer, Klipspringer) and feloideae species (domestic cat, Siberian tiger, fosa). Characteristic "fingerprints" are obtained from the positive ion spectra that allow the differentiation between both animal groups. In contrast to the lipid extracts of ruminantia spermatozoa which predominantly contain ether lipids including essential amounts of plasmalogens, the more complex phospholipid composition of feloideae spermatozoa is clearly dominated by diacyl phospholipids and contains only marginal amounts of plasmalogens. It will also be shown that the lipid compositions of ejaculated, electroejaculated and cauda epididymal spermatozoa of the same species are very similar and give comparable data. Therefore, the analysis of ejaculated spermatozoa is not an absolute must. (C) 2009 Published by Elsevier Inc.