Control of the Na+/Ca2+ exchanger 3 promoter by cyclic adenosine monophosphate and Ca2+ in differentiating neurons
Control of the Na+/Ca2+ exchanger 3 promoter by cyclic adenosine monophosphate and Ca2+ in differentiating neurons
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分化神经元中环磷酸腺苷和 Ca2+ 对 Na /Ca2 交换器 3 启动子的控制
DOI：
10.1046/j.1471-4159.2003.01511.x
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发表时间：
2003
影响因子：
4.7
通讯作者：
E. Carafoli
中科院分区：
文献类型：
--
作者：
N. Gabellini;S. Bortoluzzi;G. Danieli;E. Carafoli
人类溶质载体家族8成员3基因（SLC8A3）编码Na+/Ca2+交换异构体3 (NCX3)，在染色体14q24.2上被发现。最小启动子区位于外显子1上游250bp处。转染的SH - SY5Y细胞中pGL3启动子构建的荧光素酶报告基因检测证实了这一点。在维甲酸和脑源性神经营养因子（BDNF）序贯处理诱导的细胞系分化过程中，对启动子活性进行监测。其活性由环AMP （cAMP）通过CRE （cAMP反应元件）诱导，并由维甲酸刺激。KCl介导的质膜部分去极化诱导的细胞内Ca2+的增加下调了基础转录和cAMP刺激的转录。后者的下调可能是由钙调蛋白依赖性激酶（CaMKII）介导的CRE结合蛋白磷酸化介导的。维甲酸处理后，细胞暴露于BDNF后，在最初的5小时内迅速诱导启动子活性，并在前2小时内迅速诱导CRE结合蛋白磷酸化。cAMP进一步增强了启动子活性，但对Ca2+不敏感。在BDNF刺激的细胞中，cAMP升高导致ATF1优先磷酸化，而不是CRE结合蛋白优先磷酸化。
The human gene for member 3 of solute carrier family 8 (SLC8A3), encoding the Na+/Ca2+ exchanger isoform 3 (NCX3), was identified on chromosome 14q24.2. The minimal promoter region was predicted 250 bp upstream of exon 1. This was confirmed by luciferase reporter assays of pGL3‐promoter constructs in transfected SH‐SY5Y cells. The promoter activity was monitored during the differentiation of this cell line elicited by the sequential treatment with retinoic acid and brain‐derived neurotrophic factor (BDNF). The activity was induced by cyclic AMP (cAMP) via the CRE (cAMP response element) and was stimulated by retinoic acid. The increase of intracellular Ca2+ induced by the partial depolarization of the plasma membrane with KCl down‐regulated both the basal and the cAMP‐stimulated transcription. The down‐regulation of the latter may be mediated by the phosphorylation of the CRE‐binding protein by a calmodulin‐dependent kinase (CaMKII). The exposure of cells to BDNF after treatment with retinoic acid rapidly induced promoter activity during the initial five hours and phosphorylation of CRE‐binding protein during the first two hours. The promoter activity was further enhanced by cAMP, but became insensitive to Ca2+. In BDNF‐stimulated cells cAMP elevation caused the preferential phosphorylation of ATF1 instead of that of CRE‐binding protein.
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DOI：
--
发表时间：
1998-12
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
M. Anderson;A. Braun;Yuejin Wu;T. Lu;H. Schulman;R. Sung
通讯作者：
M. Anderson;A. Braun;Yuejin Wu;T. Lu;H. Schulman;R. Sung
影响因子：
5.8
作者：
Bhatt, HS;Conner, BP;Easom, RA
通讯作者：
Easom, RA
影响因子：
56.9
作者：
SHENG, M;THOMPSON, MA;GREENBERG, ME
通讯作者：
GREENBERG, ME
影响因子：
--
作者：
KOFUJI, P;HADLEY, RW;SCHULZE, DH
通讯作者：
SCHULZE, DH