Assessment of the use of compost stability as an indicator of alkane and aromatic hydrocarbon degrader abundance in green waste composting materials and finished composts for soil bioremediation application.

Assessment of the use of compost stability as an indicator of alkane and aromatic hydrocarbon degrader abundance in green waste composting materials and finished composts for soil bioremediation application.
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评估使用堆肥稳定性作为绿色废物堆肥材料和土壤生物修复应用成品堆肥中烷烃和芳香烃降解剂丰度的指标。

DOI:
10.1016/j.wasman.2019.06.030
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发表时间:
2019
期刊:
Waste management (New York, N.Y.)
影响因子:
--
通讯作者:
Guillen Ferrari D
Guillen Ferrari D
中科院分区:
--
文献类型:
--
作者:
Guillen Ferrari D

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从不同的商业异地堆肥场收集了绿色垃圾堆肥材料和成品堆肥,这些垃圾都是处理源分离的绿色垃圾原料。使用标准的ORG0020动态呼吸试验测定每种材料的稳定性。为了评估稳定性是否可以作为绿色垃圾堆肥材料和成品堆肥作为土壤生物修复改良剂的潜在适宜性的指标,将其与用定量聚合酶链式反应(QPCR)方法测定的烷烃和芳烃降解物的丰度进行了比较。特别是针对Gram阳性(GP)和GN(GN)群体的碱带、多芳烃环羟化双加氧酶基因(PAH-RHD)的引物被用来进行qPCR分析。结果表明,堆肥稳定性与基因丰度之间没有直接的相关性。此外,alkB基因丰度的增加与PAH-RHD基因丰度无关。这一结果支持使用定量聚合酶链式反应作为一种工具,逐个地点地筛选有机改良剂用于土壤生物修复处理。
Green waste composting materials and finished composts were collected from different commercial ex situ composting sites all treating source segregated green waste feedstocks. Stability of each material was determined using the standard ORG0020 dynamic respiration test. To assess whether stability could be used as an indicator for the potential suitability of green waste composting materials and finished composts as amendments for soil bioremediation, comparison was made with alkane and aromatic hydrocarbon degrader abundance determined using a quantitative PCR (qPCR) approach. Specifically, primers targetingalkBand, polyaromatic hydrocarbon ring-hydroxylating dioxygenases genes (PAH-RHD) of Gram positive (GP) and Gram negative (GN) populations were used for qPCR analysis. The results showed no direct correction between compost stability and gene abundance. Further, increase inalkBgene abundance was not linked to PAH-RHD gene abundance. The results support the use of qPCR as a tool for screening organic amendments on a site by site basis for soil bioremediation treatment.
餐厨垃圾堆肥修复重度燃油污染土壤及其挥发性有机化合物(VOC)排放
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发表时间: 2018
影响因子: --
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发表时间: 2017
期刊:
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DOI: --
发表时间: 2015
期刊: Waste Management
影响因子: 8.1
作者:
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