MuERV-L is one of the earliest transcribed genes in mouse one-cell embryos
MuERV-L is one of the earliest transcribed genes in mouse one-cell embryos
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DOI：
10.1095/biolreprod.102.007906
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发表时间：
2003-02-01
影响因子：
3.6
通讯作者：
Imai, H
中科院分区：
文献类型：
--
作者：
Kigami, D;Minami, N;Imai, H
研究了小鼠内源性逆转录病毒样(MERV-L)基因在小鼠着床前胚胎中的表达模式和功能。L病毒在第一个细胞周期中从S期开始(受精后8h)快速转录。受精卵基因组转录被a-Amanitin抑制后，MuERV-L的表达被完全抑制。这些结果表明，L病毒是从受精卵基因组转录而来的，并且比以前报道的任何其他基因都更早表达。此外，MERV-L在第一轮DNA合成被抑制的情况下仍有表达，表明其表达受受精卵时钟的控制。用反义寡核苷酸检测了小鼠逆转录病毒L在小鼠胚胎发育中的作用。用反义寡核苷酸处理4-细胞期后的胚胎，胚胎的发育能力明显受到抑制。这一结果表明，MuERVL在小鼠胚胎着床前的早期发育中起着重要作用。
The expression pattern and function of the murine endogenous retrovirus-like (MuERV-L) gene in mouse preimplantation embryos was investigated. MuERV-L was rapidly transcribed from the beginning of S phase (8 h after fertilization) in the first cell cycle. MuERV-L expression was completely repressed when transcription from the zygotic genome was inhibited by a-amanitin. These results reveal that MuERV-L is transcribed from the zygotic genome and that it is expressed earlier than any other genes previously reported. In addition, MuERV-L was expressed even when the first round of DNA synthesis was inhibited by aphidicolin, suggesting that its expression is controlled by the zygotic clock. The function of MuERV-L in the development of mouse embryos was also examined using antisense oligonucleotides. The developmental competence of embryos was markedly suppressed after the 4-cell stage when they were treated with antisense oligonucleotides. This result suggests that MuERVL plays an important role in the development of mouse embryos at the early preimplantation stage.