The Gut Microbiota of Healthy Aged Chinese Is Similar to That of the Healthy Young.
The Gut Microbiota of Healthy Aged Chinese Is Similar to That of the Healthy Young.
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DOI：
10.1128/msphere.00327-17
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发表时间：
2017-09
期刊：
影响因子：
4.8
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Bian G;Gloor GB;Gong A;Jia C;Zhang W;Hu J;Zhang H;Zhang Y;Zhou Z;Zhang J;Burton JP;Reid G;Xiao Y;Zeng Q;Yang K;Li J
我们报告了大规模使用组成数据分析来建立中国人群中一个非常健康的队列中的基线微生物群组成。该基线将用于未来慢性或急性疾病队列的比较。除了儿童和成人的微生物群的预期差异外，我们发现这一人群中老年人的微生物群几乎在所有方面都与同一人群中年轻数十岁的健康人相似。我们推测，这种相似性是积极健康的生活方式和饮食的结果，虽然因果关系不能归因于这个（或任何其他）横截面设计。一个令人惊讶的结果是，20多岁的人的肠道微生物群与其他年龄组的人不同，并且这一结果被复制，表明这是一个可重复的发现，与其他人群不同。老年人的微生物群被不同地描述为比年轻群体的微生物群或多或少地多样化，但是所使用的比较组和老年人群的定义在实验之间不同。这些差异通常由零假设统计检验来描述，这在处理大的多变量样本时是众所周知的不可重复的。我们收集并检查了1,000多名非常健康的中国人的肠道微生物群，这些人的年龄从3岁到100岁以上。16 S rRNA基因测序结果的分析使用了一个组成数据分析范式，再加上措施的效果大小，其中排序，差异丰度和相关性可以探索和分析在一个统一的和可重复的框架。我们的分析显示，与其他队列相比，有几个令人惊讶的结果。首先，健康老年组的总体微生物群组成与年轻几十岁的人相似。其次，肠道菌群分布的主要差异在20岁之前被发现。第三，从30岁到>100岁的个体之间的肠道微生物群差异很小。第四，男性的肠道微生物群似乎比女性更多变。总的来说，目前的研究结果表明，在这项横断面研究中，健康老年人的微生物群与同一人群中健康年轻人的微生物群几乎没有什么不同，尽管确实存在的微小变化取决于比较队列。我们报告了大规模使用成分数据分析来建立中国人群中一个非常健康的队列中的基线微生物群组成。该基线将用于未来慢性或急性疾病队列的比较。除了儿童和成人的微生物群的预期差异外，我们发现这一人群中老年人的微生物群几乎在所有方面都与同一人群中年轻数十岁的健康人相似。我们推测，这种相似性是积极健康的生活方式和饮食的结果，虽然因果关系不能归因于这个（或任何其他）横截面设计。一个令人惊讶的结果是，20多岁的人的肠道微生物群与其他年龄组的人不同，并且这一结果被复制，表明这是一个可重复的发现，与其他人群不同。
We report the large-scale use of compositional data analysis to establish a baseline microbiota composition in an extremely healthy cohort of the Chinese population. This baseline will serve for comparison for future cohorts with chronic or acute disease. In addition to the expected difference in the microbiota of children and adults, we found that the microbiota of the elderly in this population was similar in almost all respects to that of healthy people in the same population who are scores of years younger. We speculate that this similarity is a consequence of an active healthy lifestyle and diet, although cause and effect cannot be ascribed in this (or any other) cross-sectional design. One surprising result was that the gut microbiota of persons in their 20s was distinct from those of other age cohorts, and this result was replicated, suggesting that it is a reproducible finding and distinct from those of other populations. The microbiota of the aged is variously described as being more or less diverse than that of younger cohorts, but the comparison groups used and the definitions of the aged population differ between experiments. The differences are often described by null hypothesis statistical tests, which are notoriously irreproducible when dealing with large multivariate samples. We collected and examined the gut microbiota of a cross-sectional cohort of more than 1,000 very healthy Chinese individuals who spanned ages from 3 to over 100 years. The analysis of 16S rRNA gene sequencing results used a compositional data analysis paradigm coupled with measures of effect size, where ordination, differential abundance, and correlation can be explored and analyzed in a unified and reproducible framework. Our analysis showed several surprising results compared to other cohorts. First, the overall microbiota composition of the healthy aged group was similar to that of people decades younger. Second, the major differences between groups in the gut microbiota profiles were found before age 20. Third, the gut microbiota differed little between individuals from the ages of 30 to >100. Fourth, the gut microbiota of males appeared to be more variable than that of females. Taken together, the present findings suggest that the microbiota of the healthy aged in this cross-sectional study differ little from that of the healthy young in the same population, although the minor variations that do exist depend upon the comparison cohort. IMPORTANCE We report the large-scale use of compositional data analysis to establish a baseline microbiota composition in an extremely healthy cohort of the Chinese population. This baseline will serve for comparison for future cohorts with chronic or acute disease. In addition to the expected difference in the microbiota of children and adults, we found that the microbiota of the elderly in this population was similar in almost all respects to that of healthy people in the same population who are scores of years younger. We speculate that this similarity is a consequence of an active healthy lifestyle and diet, although cause and effect cannot be ascribed in this (or any other) cross-sectional design. One surprising result was that the gut microbiota of persons in their 20s was distinct from those of other age cohorts, and this result was replicated, suggesting that it is a reproducible finding and distinct from those of other populations.