Nucleotide sequence at the insertion sites of a kanamycin transposon
Nucleotide sequence at the insertion sites of a kanamycin transposon
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卡那霉素转座子插入位点的核苷酸序列
作者：
A. Oka;N. Nomura;K. Sugimoto;H. Sugisaki;M. Takanami
携带卡那霉素抗性基因的TRANSPOSON Tn 903大小为3，100个碱基对，在两端含有1，050个碱基对的反向重复序列1 -4。我们在这里报告的9个碱基对的重复序列的插入位点的Tn 903的生成。Tn 903可以转座到大肠杆菌噬菌体fd DNA 1，2上的至少9个不同位点。我们还将Tn 903转座到约1，600个碱基对的小大肠杆菌素E1质粒（ColE 1）衍生物上的三个位点。在小受体DNA分子上存在这些许多插入位点意味着在受体上的靶位点中不涉及特异性序列。为了在分子水平上研究Tn 903转座的机制，我们现在分析了Tn 903在小ColE 1 DNA分子上的三个独立插入。三个靶位点的核苷酸序列和Tn 903和小ColE 1之间的相应连接的核苷酸序列已经确定。
THE TRANSPOSON Tn903 carrying a gene for kanamycin resistance, is 3,100 base pairs in size and contains an inverted repeat sequence of 1,050 base pairs at both ends1–4. We report here the generation of a 9-base pair repeated sequence at the insertion site of Tn903. Tn903 can be transposed to at least nine different sites on the coliphage fd DNA1,2. We have also transposed Tn903 to three sites on small colicin E1 plasmid (ColE1) derivatives of about 1,600 base pairs. The presence of these many insertion sites on small recipient DNA molecules implies that no specific sequence is involved in the target sites on the recipient. To investigate the mechanism of transposition of Tn903 at the molecular level, we have now analysed three independent insertions of Tn903 on the small ColE1 DNA molecules. The nucleotide sequences of the three target sites and of the corresponding junctions between Tn903 and the small ColE1 have been determined.