Oviposition but Not Sex Allocation Is Associated with Transcriptomic Changes in Females of the Parasitoid Wasp Nasonia vitripennis.
Oviposition but Not Sex Allocation Is Associated with Transcriptomic Changes in Females of the Parasitoid Wasp Nasonia vitripennis.
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DOI：
10.1534/g3.115.021220
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发表时间：
2015-10-28
期刊：
影响因子：
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通讯作者：
Shuker DM
中科院分区：
文献类型：
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作者：
Cook N;Trivedi U;Pannebakker BA;Blaxter M;Ritchie MG;Tauber E;Sneddon T;Shuker DM
将表现型的进化与潜在的基因型联系起来是进化遗传学的一个关键目标，对我们理解自然选择如何塑造特征至关重要。在这里，我们用转录组学的方法研究了拟寄生蜂蜂（Nasonia vitripennis）性别分配行为的遗传基础。雌性根据局部配偶竞争（LMC）理论分配后代性别。当一只或几只雌性在一块土地上产卵时，就会产生偏向雌性的性别比例。随着为一个种群贡献后代的雌性数量的增加，较少偏向雌性的性别比例受到青睐。我们对比了雌性在已知影响性别分配的条件下产卵时的转录组反应：产卵者数量（社会线索）和宿主状态（寄生与否）。我们发现，当雌性在贴片上遇到其他雌性或与产卵者评估宿主质量时，性别分配的变化与全身基因表达的显著变化无关。我们还发现，雌性在响应宿主提示和社会提示而进行性分配时产生的基因表达变化是非常密切相关的。我们扩大了与纳索尼亚产卵行为相关的候选基因列表，其中一些基因可能参与了同时性分配能力的基本过程，包括精子的储存和利用。
Linking the evolution of the phenotype to the underlying genotype is a key aim of evolutionary genetics and is crucial to our understanding of how natural selection shapes a trait. Here, we consider the genetic basis of sex allocation behavior in the parasitoid wasp Nasonia vitripennis using a transcriptomics approach. Females allocate offspring sex in line with the local mate competition (LMC) theory. Female-biased sex ratios are produced when one or a few females lay eggs on a patch. As the number of females contributing offspring to a patch increases, less female-biased sex ratios are favored. We contrasted the transcriptomic responses of females as they oviposit under conditions known to influence sex allocation: foundress number (a social cue) and the state of the host (parasitized or not). We found that when females encounter other females on a patch or assess host quality with their ovipositors, the resulting changes in sex allocation is not associated with significant changes in whole-body gene expression. We also found that the gene expression changes produced by females as they facultatively allocate sex in response to a host cue and a social cue are very closely correlated. We expanded the list of candidate genes associated with oviposition behavior in Nasonia, some of which may be involved in fundamental processes underlying the ability to facultatively allocate sex, including sperm storage and utilization.