Sedative and Anxiolytic Activities of Opuntia ficus indica (L.) Mill.: An Experimental Assessment in Mice
Sedative and Anxiolytic Activities of Opuntia ficus indica (L.) Mill.: An Experimental Assessment in Mice
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DOI：
10.3390/molecules25081844
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发表时间：
2020-04-02
期刊：
影响因子：
4.6
通讯作者：
Sobarzo-Sanchez, Eduardo
中科院分区：
文献类型：
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作者：
Akkol, Esra Kupeli;Ilhan, Mert;Sobarzo-Sanchez, Eduardo
民族植物学野外调查揭示了对印度仙人掌果实的利用。轧机。用于治疗糖尿病、烧伤、支气管哮喘、便秘、肾结石和风湿性疼痛，并在土耳其民间医学中用作镇静剂。本研究旨在通过实验验证无花果果实的功效，并采用生物测定指导方法确定其活性成分。将果实粗甲醇提取物依次分离成正己烷、二氯甲烷、乙酸乙酯、正丁醇和水五个亚提取物。对活性最高的乙酸乙酯（EtOAc）亚提取物进行进一步实验，并在Sephadex LH-20上进行连续柱层析。为了进行活性评估，将每个提取物或馏分提交给生物测定系统；镇静、抗焦虑作用采用牵引试验、壁炉试验、孔板试验、高架+迷宫试验、开场试验，催眠作用采用硫喷妥诱发睡眠试验。从甲醇提取物中得到的亚提取物中，乙酸乙酯亚提取物在生物测定系统中表现出显著的镇静和抗焦虑作用。从乙酸乙酯亚提取物中分离得到主要成分，并利用波谱技术鉴定其结构为异鼠李素、异鼠李素3- o -糖苷、异鼠李素3- o -芦丁苷和山奈酚3- o -芦丁苷。总之，这项研究证实了土耳其民间医学中使用这种植物对抗焦虑的说法。
Ethnobotanical field surveys revealed the use of fruits of Opuntia ficus indica (L.) Mill. for treating diabetes, burns, bronchial asthma, constipation, kidney stones, and rheumatic pains and as a sedative in Turkish folk medicine. This study aimed to verify the efficacy of the fruits of O. ficus indica experimentally and to define components responsible for the activity using bioassay-guided procedures. The crude methanolic extract of the fruits was sequentially fractionated into five subextracts: n-hexane, dichloromethane, ethyl acetate, n-butanol, and water. Further experiments were carried out on the most active subextract, that is, the ethyl acetate (EtOAc) subextract, which was further subjected to fractionation through successive column chromatographic applications on Sephadex LH-20. For activity assessment, each extract or fraction was submitted to bioassay systems; traction test, fireplace test, hole-board test, elevated plus-maze test, and open-field test were used for sedative and anxiolytic effects, and a thiopental-induced sleeping test was used for the hypnotic effect. Among the subextracts obtained from the methanolic extract, the EtOAc subextract showed significant sedative and anxiolytic effects in the bioassay systems. From the EtOAc subextract, major components were isolated, and their structures were determined as isorhamnetin, isorhamnetin 3-O-glucoside, isorhamnetin 3-O-rutinoside, and kaempferol 3-O-rutinoside using spectral techniques. In conclusion, this study confirmed the claimed use of the plant against anxiety in Turkish folk medicine.