Effect of vimentin on reactive gliosis:: In vitro and in vivo analysis
Effect of vimentin on reactive gliosis:: In vitro and in vivo analysis
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DOI：
10.1089/0897715042441819
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发表时间：
2004-11-01
影响因子：
4.2
通讯作者：
Cai, WQ
中科院分区：
文献类型：
--
作者：
Lin, JK;Cai, WQ
反应性神经胶质增生是中枢神经系统（CNS）多种损伤的主要结果，并导致胶质瘢痕的形成，从而阻碍轴突的再生。中间丝蛋白波形蛋白在CNS的病理学中被发现，主要在CNS损伤的附近。在本研究中，我们研究了波形蛋白在胶质瘢痕形成中的作用，在体外和体内，通过免疫组织化学，Western印迹分析，原位杂交。体外实验表明，波形蛋白和胶质细胞酸性蛋白（GFAP）的免疫荧光标记的强度一致降低后，转染逆转录病毒携带反义互补DNA（cDNA）的波形蛋白的星形胶质细胞。转染后星形胶质细胞的生长受到抑制，波形蛋白mRNA表达减少。体内研究表明，逆转录病毒转染反义vimentin基因可抑制大鼠脑刺伤后vimentin和GFAP的表达。这些结果表明，波形蛋白可能在中枢神经系统胶质瘢痕的形成中发挥关键作用。
Reactive gliosis is a prominent result of many types of insult to the central nervous system (CNS) and leads to the formation of glial scar that impedes the regeneration of axons. The intermediate filament protein vimentin is found in pathology of the CNS, mainly in the vicinity of injuries to the CNS. In the present study we investigated the role of vimentin in the formation of glial scars in vitro and in vivo by using immunohistochemistry, Western blot analysis, and in situ hybridization. In vitro experiments showed that the intensity of immunofluorescent labeling for vimentin and glial fibrillary acidic protein (GFAP) was consistently decreased in astrocytes after transfection with a retrovirus carrying antisense complementary DNA (cDNA) for vimentin. Transfection also inhibited the growth of astrocytes and decreased the expression of vimentin mRNA. In vivo studies demonstrated that transfection with the retrovirus carrying the antisense cDNA vimentin inhibited the upregulation of vimentin and GFAP in stab wounds in rat cerebrum. These results suggest that vimentin may play a key role in the formation of glial scars in the CNS.