Nucleolar localization of human methionyl-tRNA synthetase and its role in ribosomal RNA synthesis.
Nucleolar localization of human methionyl-tRNA synthetase and its role in ribosomal RNA synthesis.
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DOI：
10.1083/jcb.149.3.567
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发表时间：
2000-05-01
期刊：
影响因子：
--
通讯作者：
Kim S
中科院分区：
文献类型：
--
作者：
Ko YG;Kang YS;Kim EK;Park SG;Kim S
人氨酰-tRNA合成酶（ARSs）通常位于细胞质中，参与蛋白质合成。在本工作中，我们发现人甲硫氨酰-tRNA合成酶（MRS）在增殖期细胞中转位到核仁，而在静止期细胞中消失。MRS的核仁定位由多种生长因子如胰岛素、PDGF和EGF触发。核仁中MRS的存在依赖于核仁中RNA的完整性和RNA聚合酶I的活性。核糖体RNA的合成，特别是减少处理的抗MRS抗体所确定的核运行试验和免疫染色后，将Br-UTP到新生的RNA与抗Br抗体。因此，人MRS在核仁中rRNA的生物合成中起作用，而它在细胞质中催化参与蛋白质合成。
Human aminoacyl–tRNA synthetases (ARSs) are normally located in cytoplasm and are involved in protein synthesis. In the present work, we found that human methionyl–tRNA synthetase (MRS) was translocated to nucleolus in proliferative cells, but disappeared in quiescent cells. The nucleolar localization of MRS was triggered by various growth factors such as insulin, PDGF, and EGF. The presence of MRS in nucleoli depended on the integrity of RNA and the activity of RNA polymerase I in the nucleolus. The ribosomal RNA synthesis was specifically decreased by the treatment of anti-MRS antibody as determined by nuclear run-on assay and immunostaining with anti-Br antibody after incorporating Br-UTP into nascent RNA. Thus, human MRS plays a role in the biogenesis of rRNA in nucleoli, while it is catalytically involved in protein synthesis in cytoplasm.
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影响因子：
7.8
作者：
DANG, CV;YANG, DCH;POLLARD, TD
通讯作者：
POLLARD, TD
DOI：
10.1111/j.1432-1033.1983.tb07244.x
发表时间：
1983-01-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
MIRANDE, M;CIRAKOGLU, B;WALLER, JP
通讯作者：
WALLER, JP
影响因子：
4.8
作者：
Park, SG;Jung, KH;Shiba, K
通讯作者：
Shiba, K
DOI：
10.1073/pnas.83.21.8054
发表时间：
1986-11-01
影响因子：
11.1
作者：
KOYASU, S;NISHIDA, E;YAHARA, I
通讯作者：
YAHARA, I
影响因子：
4.8
作者：
Giraudo, E;Primo, L;Bussolino, F
通讯作者：
Bussolino, F