Fractionated irradiation augments inter-strain variation of skin reactions among three strains of mice
Fractionated irradiation augments inter-strain variation of skin reactions among three strains of mice
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DOI：
10.1269/jrr.45.515
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发表时间：
2004-12-01
影响因子：
2
通讯作者：
Imai, T
中科院分区：
文献类型：
--
作者：
Ohta, T;Iwakawa, M;Imai, T
常规临床放射治疗中常用的多分割方案在很大程度上是基于放射生物学实验。然而，没有关于皮肤反应的实验报告（侧重于菌株间差异）显示与分次给药方案的临床相关性。本研究以A/J、C57 BL/6 J和C3 H/HeMs近交系小鼠为研究对象，探讨不同品系小鼠多次照射后的辐射损伤差异。每天以30-60戈伊总剂量的分级剂量进行照射，其中10个部分为3-6戈伊。照射后的急性皮肤反应评分为照射后50天。将一剂药物分成几份，显然可以避免三种小鼠的皮肤损伤。没有小鼠表现出超过1.5的皮肤损伤评分，而单次剂量照射导致皮肤损伤评分高达3。然而，这三种不同的菌株，表现出不同的敏感性分次照射范围内1.5。C3 H/HeMs在40戈伊总剂量照射后未出现任何皮肤反应，而C57 BL/6 J和A/J出现各种皮肤反应。不同剂量辐照后，不同品系间的损伤潜伏期也不同，说明遗传因素导致了分次辐照后损伤程度和损伤潜伏期的明显差异。
The multifraction regimens commonly used in conventional clinical radiotherapy are largely based on radiobiological experiments. However, no experimental reports on skin reactions focusing on inter-strain differences have displayed clinical relevance to the fractionated dose schedule. In this study, mice of inbred strains A/J, C57BL/6J, and C3H/HeMs were used to reveal inter-strain difference after multifractionated irradiation. Irradiation was performed daily at graded doses of 30-60 Gy total doses, with 10 fractions of 3-6 Gy. Acute skin reactions following irradiation were scored for 50 days after irradiation. Dividing a dose into a number of fractions obviously spared skin damage in the three strains of mice. No mouse exhibited a skin damage score more than 1.5, while single dose irradiation resulted in skin damage scores up to 3. The three different strains, however, showed varying susceptibility to fractionated irradiation within the range under 1.5. C3H/HeMs did not display any skin reaction after irradiation with 40 Gy total dose, while C57BL/6J and A/J demonstrated various skin reactions. Different latent periods of damage were also observed among the strains after irradiation at each dose.Our data suggest that genetic factors cause obvious variations in severity of damage and latent period after fractionated irradiation.