Tritium toxicity: effect of low-level 3HOH exposure on developing female germ cells in the mouse.
Tritium toxicity: effect of low-level 3HOH exposure on developing female germ cells in the mouse.
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氚毒性：低水平 3HOH 暴露对小鼠雌性生殖细胞发育的影响。
DOI：
10.2307/3573952
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发表时间：
1974
影响因子：
3.4
通讯作者：
M. F. Cooper
中科院分区：
文献类型：
--
作者：
R. Dobson;M. F. Cooper
Dobson，R.L.和Cooper，M.F.氚毒性：低水平3HOH暴露对小鼠雌性生殖细胞发育的影响。辐射。第58、91-100号决议(1974)。成年雌性小鼠在受精当天注射3HOH，以使体内水中的氚含量迅速达到8.5juCi/ml、0.85juCi/ml和0.085 juCi/ml，并开始饮用每毫升含131.3UCl3HOH的饮用水，以在整个怀孕和哺乳期保持这些水平。它们的雌性后代从受孕开始就暴露在氚中，在14日龄时被处死，并在卵巢的连续切片中计数卵母细胞。这些小鼠的初级卵母细胞在8.5uci/ml的水平下比对照组减少了90%以上，在0.85uci/ml的水平下减少了30%，在0.085，ci/ml的水平，这一水平大大低于先前报道的对哺乳动物产生可测量的生物学效应的水平，仍然观察到了显著的下降。没有发现门槛的证据。数据表明，到14天大的时候，在发育过程中持续暴露的雌性小鼠的生殖细胞数量有望减少50%，每毫升体内水中的氚含量约为2.0uci。
DOBSON, R. L., AND COOPER, M. F. Tritium Toxicity: Effect of Low-Level 3HOH Exposure on Developing Female Germ Cells in the Mouse. Radiat. Res. 58, 91-100 (1974). Adult female mice were injected with 3HOH on the day of fertilization to give prompt levels of tritium in body water of 8.5, 0.85, and 0.085 juCi/ml and started on drinking water containing 13, 1.3, and 0.13 ,uCi 3HOH per ml to maintain these levels throughout pregnancy and lactation. Their female offspring, thus exposed to tritium from conception, were sacrificed at 14 days of age, and oocytes were counted in serial sections of ovaries. Primary oocytes in these mice were decreased below control numbers by more than 90% at the 8.5 ,uCi/ml level and 30% at 0.85 /lCi/ml. At 0.085 ,Ci/ml, a level substantially below those reported previously to cause measurable biological effects in mammals, a significant decrease was still observed. No evidence of a threshold was found. The data indicate that, by 14 days of age, a 50% decrease in the number of germ cells can be expected in a female mouse exposed continuously during development to approximately 2 ,uCi of tritium per ml body water.