Time course alterations of virus sequences and immunoglobulin titers in a chronic hepatitis E patient
Time course alterations of virus sequences and immunoglobulin titers in a chronic hepatitis E patient
复制标题
慢性戊型肝炎患者病毒序列和免疫球蛋白滴度的时程变化
DOI：
10.1111/hepr.13480
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发表时间：
2020
影响因子：
4.2
通讯作者：
Asahina Yasuhiro
中科院分区：
文献类型：
--
作者：
Nitta Sayuri;Takahashi Kazuaki;Kawai‐Kitahata Fukiko;Tsuchiya Jun;Sato Ayako;Miyoshi Masato;Murakawa Miyako;Itsui Yasuhiro;Nakagawa Mina;Azuma Seishin;Kakinuma Sei;Watanabe Mamoru;Asahina Yasuhiro
目的戊型肝炎病毒（HEV）可引起免疫功能低下的宿主慢性感染.然而，HEV在持续感染期间的动力学尚不清楚。为了阐明持续感染过程中病毒序列和抗HEV抗体的时程变化，我们分析了慢性戊型肝炎患者的HEV序列和抗HEV抗体滴度。采用酶免疫法检测血清样本中抗HEV抗体（免疫球蛋白A、免疫球蛋白M和免疫球蛋白G）的滴度。鉴定并比较从连续血清样本中分离的HEV的完整或接近完整的核苷酸序列。系统发育分析也performed.ResultsAlterations从慢性戊型肝炎患者的抗-HEV抗体的变化是不同于以前报道的急性戊型肝炎患者。病毒序列在未治疗期间未发生变化，但在利巴韦林治疗开始后观察到核苷酸突变。此外，该菌株的序列具有极高的同一性，从猪肝脏中分离在Japan.ConclusionsVirus变异后，出现了病毒唑治疗开始。对于RBV治疗3个月后病毒仍未清除的慢性戊型肝炎患者，序列分析可能有助于决定治疗策略，并推断感染的来源。这份报告提供了深入了解戊型肝炎的慢性化，以及持续感染和利巴韦林治疗对病毒突变出现的影响。
AimHepatitis E virus (HEV) can cause chronic infection in immunocompromised hosts. However, the dynamics of HEV during persistent infection is not well understood. To elucidate time course alterations in virus sequences and anti‐HEV antibodies during persistent infection, we analyzed the HEV sequences and titers of anti‐HEV antibodies from a chronic hepatitis E patient.MethodsSerum samples were obtained from a chronic hepatitis E patient under corticosteroid therapy for neurological disease. The titers of anti‐HEV antibodies (immunoglobulin A, immunoglobulin M, and immunoglobulin G) in serum samples were detected by enzyme immunoassay. The full or near‐full nucleotide sequences of HEV isolated from consecutive serum samples were identified and compared. Phylogenetic analysis was also performed.ResultsAlterations of anti‐HEV antibodies from a chronic hepatitis E patient were different from those previously reported in acute hepatitis E patients. The virus sequence was unchanged in the period without treatment, but nucleotide mutations were observed after ribavirin treatment was started. In addition, the sequence of this strain had extremely high identity to that isolated from swine liver in Japan.ConclusionsVirus mutations in HEV emerged after ribavirin treatment was started. Sequence analysis may useful for deciding the treatment strategy for chronic hepatitis E patients who did not eliminate the virus with 3 months of RBV treatment and inferring the origin of the infection. This report provides insights into the chronicity of hepatitis E, and the impact of persistent infection and ribavirin treatment on the emergence of virus mutations.
影响因子：
6.7
作者：
Kamar, Nassim;Marion, Olivier;Dalton, Harry R.
通讯作者：
Dalton, Harry R.
影响因子：
29.4
作者：
Kamar, Nassim;Rostaing, Lionel;Izopet, Jacques
通讯作者：
Izopet, Jacques
影响因子：
4.9
作者：
Lhomme, Sebastien;Kamar, Nassim;Izopet, Jacques
通讯作者：
Izopet, Jacques