Ginsenoside Rg3 improves cardiac mitochondrial population quality: Mimetic exercise training
Ginsenoside Rg3 improves cardiac mitochondrial population quality: Mimetic exercise training
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人参皂苷 Rg3 改善心脏线粒体群质量：模拟运动训练
DOI：
10.1016/j.bbrc.2013.10.039
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发表时间：
2013-11-08
影响因子：
3.1
通讯作者：
Shen, Weili
中科院分区：
文献类型：
--
作者：
Sun, Mengwei;Huang, Chenglin;Shen, Weili
新的证据表明，运动训练可以通过改善线粒体动力学来调节线粒体质量控制。人参皂苷Rg 3（Rg 3）是人参中的活性成分之一，在草药中作为滋补剂和恢复剂是众所周知的。然而，Rg 3有益作用的分子机制一直难以捉摸。在本研究中，我们比较了人参皂甙Rg 3管理与有氧运动对心肌组织线粒体适应性的影响。研究了三组SD大鼠：（1）久坐对照组，（2）Rg 3治疗组和（3）有氧运动训练组。有氧运动训练和补充Rg 3均能增强心肌中过氧化物酶体增殖物激活受体辅激活因子I α（PGC-1 α）和核因子E2相关因子2（Nrf 2）的蛋白水平。PGC-1 α的激活导致线粒体转录因子A（Tfam）和核相关因子1（Nrf 1）的mRNA水平增加，这些变化伴随着线粒体DNA拷贝数和复合蛋白水平的增加，而Nrf 2的激活增加了II相解毒酶的水平，包括烟酰胺腺嘌呤二核苷酸磷酸：醌氧化还原酶1（NQO 1），超氧化物歧化酶（MnSOD）和过氧化氢酶。有氧运动还增强了线粒体自噬途径的活性，包括增加LC 3-I向LC 3-II的转化以及增加beclin 1和自噬相关蛋白7（ATG 7）的表达，有氧运动的这些作用与Rg 3相当。这些结果表明，Rg 3模拟物通过调节线粒体动态重塑和提高线粒体的数量和质量来改善心脏对运动的适应性。(C)2013 Elsevier Inc. All rights reserved.
Emerging evidence indicates exercise training could mediate mitochondrial quality control through the improvement of mitochondrial dynamics. Ginsenoside Rg3 (Rg3), one of the active ingredients in Panax ginseng, is well known in herbal medicine as a tonic and restorative agent. However, the molecular mechanism underlying the beneficial effects of Rg3 has been elusive. In the present study, we compared the effects of Rg3 administration with aerobic exercise on mitochondrial adaptation in cardiac muscle tissue of Sprague-Dawley (SD) rats. Three groups of SD rats were studied: (1) sedentary control, (2) Rg3-treated and (3) aerobic exercise trained. Both aerobic exercise training and Rg3 supplementation enhanced peroxisome proliferator-activated receptor coactivator I alpha (PGC-1 alpha) and nuclear factor-E2-related factor 2 (Nrf2) protein levels in cardiac muscle. The activation of PGC-1 alpha led to increased mRNA levels of mitochondrial transcription factor A (Tfam) and nuclear related factor l(Nrf1), these changes were accompanied by increases in mitochondrial DNA copy number and complex protein levels, while activation of Nrf2 increased levels of phase II detoxifying enzymes, including nicotinamide adenine dinucleotide phosphate:quinone oxidoreductase 1(NQO1), superoxide dismutase (MnSOD) and catalase. Aerobic exercise also enhanced mitochondrial autophagy pathway activity, including increased conversion of LC3-I to LC3-II and greater expression of beclin1 and autophagy-related protein 7 (ATG7), these effects of aerobic exercise are comparable to that of Rg3. These results demonstrate that Rg3 mimics improved cardiac adaptations to exercise by regulating mitochondria dynamic remodeling and enhancing the quantity and quality of mitochondria. (C) 2013 Elsevier Inc. All rights reserved.