Seasonal Stability and Dynamics of DNA Methylation in Plants in a Natural Environment
Seasonal Stability and Dynamics of DNA Methylation in Plants in a Natural Environment
复制标题
DOI：
10.3390/genes10070544
复制
发表时间：
2019-07-01
期刊：
影响因子：
3.5
通讯作者：
Kudoh, Hiroshi
中科院分区：
文献类型：
--
作者：
Ito, Tasuku;Nishio, Haruki;Kudoh, Hiroshi
DNA甲基化被认为是一个稳定的表观遗传标记，但可能对波动的环境作出反应。然而，目前还不清楚它们在自然环境中的行为。在这里，我们分析了多年生拟南芥自然种群的单克隆全基因组DNA甲基化的季节性模式。DNA甲基化的全基因组模式基本上是稳定的，大多数重复区域在一年中都被甲基化了。虽然比例很小，但我们在基因组中检测到季节性甲基化的胞嘧啶（SeMCs）。CHH背景下的SeMCs主要在基因间区域的重复序列中检测到。相比之下，基因体CG甲基化（gbM）本身在不同季节通常是稳定的，但gbM的水平与基因RNA表达的季节性稳定性呈正相关。这些结果表明，DNA甲基化在自然环境中存在两个不同的方面：表观遗传变异的来源和稳定基因表达的表观遗传标记。
DNA methylation has been considered a stable epigenetic mark but may respond to fluctuating environments. However, it is unclear how they behave in natural environments. Here, we analyzed seasonal patterns of genome-wide DNA methylation in a single clone from a natural population of the perennial Arabidopsis halleri. The genome-wide pattern of DNA methylation was primarily stable, and most of the repetitive regions were methylated across the year. Although the proportion was small, we detected seasonally methylated cytosines (SeMCs) in the genome. SeMCs in the CHH context were detected predominantly at repetitive sequences in intergenic regions. In contrast, gene-body CG methylation (gbM) itself was generally stable across seasons, but the levels of gbM were positively associated with seasonal stability of RNA expression of the genes. These results suggest the existence of two distinct aspects of DNA methylation in natural environments: sources of epigenetic variation and epigenetic marks for stable gene expression.