miR-134 Functions as a Tumor Suppressor in Cell Proliferation and Epithelial-to-Mesenchymal Transition by Targeting KRAS in Renal Cell Carcinoma Cells
miR-134 Functions as a Tumor Suppressor in Cell Proliferation and Epithelial-to-Mesenchymal Transition by Targeting KRAS in Renal Cell Carcinoma Cells
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miR-134 通过靶向肾细胞癌细胞中的 KRAS，在细胞增殖和上皮间质转化中发挥肿瘤抑制因子的作用
DOI：
10.1089/dna.2014.2629
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发表时间：
2015-06-01
影响因子：
3.1
通讯作者：
Yin, Changjun
中科院分区：
文献类型：
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作者：
Liu, Yiyang;Zhang, Mingcong;Yin, Changjun
据报道，异常microRNA（miRNAs）有助于大多数人类恶性肿瘤的发病机制。已经发现miRNA，miR-134在肾细胞癌（RCC）中下调，但其在疾病中的功能尚不清楚。本研究旨在检测miR-134在人肾细胞癌组织中的表达，并探讨其在肾细胞癌细胞系中的功能。实时定量聚合酶链反应（qPCR）用于定量人RCC样本中的miR-134。进行细胞周期、活力、迁移和侵袭的测定以评估RCC细胞中的表型变化。进行荧光素酶报告基因测定以确认KRAS（Kirsten大鼠肉瘤病毒癌基因同源物）是否是miR-134的直接靶标。Western blot用于鉴定影响RCC细胞生长和上皮-间质转化（EMT）标志物改变的潜在信号通路，其影响miR-134的转移。发现miR-134在RCC样本中下调（p
Aberrant microRNAs (miRNAs) are reported to contribute to the pathogenesis of most human malignancies. The miRNA, miR-134, has been found to be downregulated in renal cell carcinoma (RCC), but its function in the disease is unknown. The aims of this study were to detect the expression of miR-134 in human RCC samples and explore its function in RCC cell lines. Real-time qualitative polymerase chain reaction (qPCR) was used to quantify miR-134 in human RCC samples. Assays for cell cycle, viability, migration, and invasion were performed to assess the phenotypic changes in RCC cells. A luciferase reporter assay was carried out to confirm whether KRAS (Kirsten rat sarcoma viral oncogene homolog) is a direct target of miR-134. Western blot was used to identify the potential signaling pathways that had an impact on RCC cell growth and alterations of markers for epithelial-mesenchymal transition (EMT), which affected metastasis by miR-134. miR-134 was found to be downregulated in RCC samples (p