INCREASED CYTOKINE SECRETION BY HUMAN BONE-MARROW CELLS AFTER MENOPAUSE OR DISCONTINUATION OF ESTROGEN REPLACEMENT
INCREASED CYTOKINE SECRETION BY HUMAN BONE-MARROW CELLS AFTER MENOPAUSE OR DISCONTINUATION OF ESTROGEN REPLACEMENT
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DOI：
10.1210/jc.80.11.3351
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发表时间：
1995-11-01
影响因子：
5.8
通讯作者：
PFEILSCHIFTER, J
中科院分区：
文献类型：
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作者：
BISMAR, H;DIEL, I;PFEILSCHIFTER, J
对人类单个核细胞和啮齿动物的研究表明，IL-1和IL-6等细胞因子可能是绝经后骨丢失的旁分泌介质。然而，绝经后妇女局部骨微环境中这些细胞因子浓度升高的假设仍未得到证实。为了解决这个问题，我在局部乳腺癌手术中从40名妇女的髂骨提取骨髓，并分析短期培养中细胞因子的释放。绝经前(n=12)和绝经后晚期(n=18)患者细胞培养上清液中细胞因子水平差异无统计学意义。2例患者在抽吸前1个月内停止雌激素替代治疗的患者(n=5)的骨髓细胞分泌的IL-1α、肿瘤坏死因子-α、IL-6、前列腺素E(2)和粒细胞-巨噬细胞集落刺激因子显著高于绝经前或绝经后晚期患者的骨髓细胞。自然绝经5年内的妇女培养物中IL-6、前列腺素E(2)和粒细胞-巨噬细胞集落刺激因子水平也升高(n=5)。我们的数据显示，雌激素撤除与人骨髓细胞释放骨吸收细胞因子的能力增加有关，并强化了这些细胞因子可能在绝经后加速骨吸收中发挥作用的假设。
Studies on circulating human mononuclear cells and rodents have suggested that cytokines such as interleukin-1 (IL-1) and IL-6 maybe paracrine mediators of postmenopausal bone loss. However, the assumption that the concentration of these cytokines is increased in the local bone microenvironment of postmenopausal women is still unproved. To address this question, me aspirated bone marrow from the iliac crest of 40 women during surgery for localized breast cancer and analyzed cytokine release in short term cultures. Cytokine levels in the cell supernatants from premenopausal (n = 12) and late postmenopausal (n = 18) subjects were not significantly different. Bone marrow cells from women two had discontinued estrogen replacement within 1 month before aspiration (n = 5) secreted significantly more IL-1 alpha, tumor necrosis factor-alpha, IL-6, prostaglandin E(2), and granulocyte-macrophage colony-stimulating factor than bone marrow cells from either premenopausal or late postmenopausal subjects. Increased levels of IL-6, prostaglandin E(2), and granulocyte-macrophage colony stimulating factor were also observed in cultures from women who were within 5 yr of natural menopause (n = 5). Our data show that estrogen withdrawal is associated with an increased potential of human bone marrow cells to release bone-resorbing cytokines and strengthen the hypothesis that these cytokines may play a role in the accelerated bone resorption after menopause.