Decreased expression of miR-150, miR146a and miR424 in type 1 diabetic patients: Association with ongoing islet autoimmunity
Decreased expression of miR-150, miR146a and miR424 in type 1 diabetic patients: Association with ongoing islet autoimmunity
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1 型糖尿病患者中 miR-150、miR146a 和 miR424 表达降低：与正在进行的胰岛自身免疫相关
DOI：
10.1016/j.bbrc.2017.06.196
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发表时间：
2018-04-06
影响因子：
3.1
通讯作者：
Yang, Tao
中科院分区：
文献类型：
--
作者：
Wang, Guofeng;Gu, Yong;Yang, Tao
背景：最近的研究揭示了 1 型糖尿病 (T1D) 等自身免疫性疾病患者的 miRNA 谱发生改变。在这里，我们进行了一项观察性研究，检测了 T1D 患者外周血单核细胞 (PBMC) 中这 5 种 miRNA（miR-150、miR-146a、miR-424、miR-181a 和 miR-142-3P）的表达水平。方法：PBMC 取自 T1D 队列 (n = 78)、2 型糖尿病 (T2D，n = 46) 和健康对照受试者（n = 56）。使用 SYBR 定量实时 PCR (qRT-PCR) 对 5 种 miRNA 进行定量分析。所有值均标准化为内源对照 U6。然后，我们比较了 T1DM 和年龄匹配的健康对照者 PBMC 中 5 种 miRNA 的表达水平，并将 miRNA 表达水平与 T1D 队列中的 β 细胞功能、自身抗体和血糖控制相关。结果：我们发现 T1D 患者中 miR-150、miR-146a 和 miR-424 降低，这可以与非糖尿病患者和 T2D 患者区分开来 (p < 0.05)。此外，与 GADA 自身抗体阴性 (Ab-) 患者相比，GADA 自身抗体阳性 (Ab+) 患者的 PBMC 显着减少。然而，年龄、性别、糖尿病病程、C 肽和血糖等特征与 miRNA 表达水平之间不存在相关性 (p > 0.05)。结论：我们的研究表明 miR-150、miR-146a 和 miR-424 可能是 T1DM 的潜在生物标志物，可应用于临床，并为该疾病的分子机制提供了新的见解。 (C) 2017 年，爱思唯尔公司出版
Background: Recent studies revealed altered miRNAs profiling in patients with autoimmune diseases, including type 1 diabetes (T1D). Here, we conducted an observational study and examined the expression levels of these five miRNAs (miR-150,miR-146a,miR-424,miR-181a and miR-142-3P) in peripheral blood mononuclear cells (PBMCs) from T1D patients.Methods: PBMCs were obtained from T1D cohorts (n = 78), type 2 diabetes (T2D, n = 46) and healthy control subjects (n = 56). Quantitative analysis of five miRNAs were performed using SYBR quantitative real time PCR (qRT-PCR). All values were normalized to endogenous control U6. Then, We compared expression level of five miRNAs in PBMCs from T1DM and age-matched healthy controls, and related the miRNAs expression levels to beta-cell function, autoantibodies and glycaemic control in T1D cohort.Results: We identified decreased miR-150, miR-146a and miR-424 in patients with T1D, which can distinguished from non-diabetic patients and T2D patients (p < 0.05). Furthermore, they were significantly decreased in PBMCs from GADA autoantibody-positive (Ab+) patients versus GADA autoantibody-negative (Ab-) patients. However, there was no correlation between characteristics such as age, sex, diabetes duration, C-peptide, and glycaemic, and the expression level of miRNAs (p > 0.05).Conclusions: Our study indicated miR-150, miR-146a and miR-424 may be potential biomarkers of T1DM with applications in the clinical setting as well as provided new insights into the molecular mechanisms involved in this disorder. (C) 2017 Published by Elsevier Inc.