Kobuvirus (Aichivirus B) infection in Brazilian cattle herds
Kobuvirus (Aichivirus B) infection in Brazilian cattle herds
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DOI：
10.1007/s11259-014-9600-7
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发表时间：
2014-06-01
影响因子：
2.2
通讯作者：
Alfieri, Amauri Alcindo
中科院分区：
文献类型：
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作者：
Ribeiro, Juliane;Lorenzetti, Elis;Alfieri, Amauri Alcindo
全世界很少有研究涉及在牛群中检测到爱知病毒B，而且这种病毒从未在南美洲被诊断出来。这项研究评估了2010年2月至2012年5月期间从巴西四个地理区域(南部、东南部、中西部和北部)收集的222份腹泻粪便样本。为了评估在不同类型的牲畜中发生的频率，我们对来自肉牛群(n=105)和奶牛群(n=117)的样本进行了评估。为了确定更容易感染的动物类别，抽样包括来自小牛(n=182)和成年动物(n=40)的样本。用RT-PCR方法从肉牛和奶牛中扩增出AichiVirus RdRp基因的216bp片段，扩增出18.2%(40/222)的样品。最高(P
There are few studies involving the detection of Aichivirus B in cattle herds worldwide, and this virus has never been diagnosed in South America. This study evaluated 222 diarrhoeic faecal samples from four Brazilian geographical regions (South, Southeast, Midwest, and North), collected between February 2010 to May 2012. To evaluate the frequency of occurrence in different types of livestock, samples from beef (n=105) and dairy (n=117) cattle herds were evaluated. To determine the category of animals more susceptible to infection, the sampling included samples from calves (n=182) and adults animals (n=40). The 216 bp fragment of the Aichivirus RdRp gene was amplified by a RT-PCR assay in 18.2 % (40/222) of the samples evaluated in both beef and dairy cattle animals. The highest (P