Surface Tunable Polymersomes Loaded with Magnetic Contrast Agent and Drug for Image Guided Cancer Therapy
Surface Tunable Polymersomes Loaded with Magnetic Contrast Agent and Drug for Image Guided Cancer Therapy
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DOI：
10.1166/jnn.2013.6979
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发表时间：
2013-03-01
影响因子：
--
通讯作者：
Park, In-Kyu
中科院分区：
文献类型：
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作者：
Muthiah, Muthunarayanan;Lee, Sang Joon;Park, In-Kyu
以聚琥珀酰亚胺(p)为原料，将带正电的聚亚胺（PEI-P）、带中性电的聚乙二醇（PEG-P）和带负电的甘氨酸（GLY-P）引入聚合物骨架聚琥珀酰亚胺(p)，合成了具有不同表面电荷的聚合体。然后用超顺磁性铁纳米粒子（SPIONs）制备聚合体，分别得到PEI-P包封SPIONs （PEI-PS）、PEG-P包封SPIONs （PEG-PS）和GLY-P包封SPIONs （GLY-PS）。动态光散射分析GLY-PS、PEG-PS和PEI-PS的平均粒径分别为163 nm、105 nm和285 nm左右。GLY-PS、PEG-PS和PEI-PS的表面电荷分别为-29.5、-18.9和+44。通过核磁共振（NMR）证实了PEI、PEG和GLY在聚合物骨架中的存在。在制备过程中，GLY-PS、PEG-PS和PEI-PS分别负载抗癌药物紫杉醇。与GLY-PS和PEI-PS相比，PEG-PS的药物释放速度更快。静脉给药GLY-PS、PEG-PS和PEI-PS后，建立小鼠体内半脾转移肝模型，并进行MRI成像。从t2加权成像来看，PEG-PS明显比其他带电荷的GLY-PS和PEI-PS更有效地在脾脏和肝脏中积累。通过本研究，可以同时有效地展示基于纳米颗粒的抗癌药物传递和成像。
Polymersomes with different surface charges were synthesized from polysuccinimide (p) by introducing positively charged polyethylenimine (PEI-P), neutrally charged polyethylene glycol (PEG-P), and negatively charged glycine (GLY-P) to the polymer backbone polysuccinimide (P). Then, the polymersomes were prepared with super paramagnetic iron nanoparticles (SPIONs) to obtain PEI-P encapsulating SPIONs (PEI-PS), PEG-P encapsulating SPIONs (PEG-PS), and GLY-P encapsulating SPIONs (GLY-PS), respectively. The average particle sizes of GLY-PS, PEG-PS, and PEI-PS were analyzed by dynamic light scattering, and it was around 163 nm, 105 nm, and 285 nm, respectively. The surface charges of GLY-PS, PEG-PS, and PEI-PS was found to be -29.5, -18.9, and +44, respectively. The presence of PEI, PEG, and GLY in the polymer backbone was confirmed with nuclear magnetic resonance (NMR). The GLY-PS, PEG-PS, and PEI-PS were loaded with the anticancer drug paclitaxel during the preparation. The drug release from the PEG-PS was faster compared to GLY-PS and PEI-PS. An in vivo hemi-spleen mouse metastatic liver model was established and imaged with MRI after intravenous administration of GLY-PS, PEG-PS, and PEI-PS. From the T2-weighted imaging, it was evident that PEG-PS accumulated in the spleen and liver more efficiently than the other charged formulations of GLY-PS and PEI-PS. From this study, the nanoparticle-based delivery and imaging of anti-cancer drugs could be effectively demonstrated simultaneously.