ALLOCATION OF C-14 CARBON IN ECTOMYCORRHIZAL WILLOW
ALLOCATION OF C-14 CARBON IN ECTOMYCORRHIZAL WILLOW
复制标题
DOI：
10.1111/j.1469-8137.1994.tb03993.x
复制
发表时间：
1994-09-01
期刊：
影响因子：
9.4
通讯作者：
TINKER, PB
中科院分区：
文献类型：
--
作者：
DURALL, DM;JONES, MD;TINKER, PB
在外生菌根群落中，碳从植物流向真菌还没有很好的量化。本研究的目的是使用C-14来量化外生菌根相对于非菌根杨柳（Salix viminalis L.）Bowles混合）。生根插条接种与Thelephora terrestris（Ehrh）。Fr.或左非菌根。非菌根植物生长在相同的（4 mg kg（-1）碳酸氢盐可提取的P）（NM-P）或更高的（21 mg kg（-1））土壤P浓度（NM+P）下，在该浓度下非菌根植物的大小与菌根（M-P）植物相似。在种植后41、51、76和89天，将芽暴露于（CO2）-C-14脉冲。在202小时追踪期后收获植物。C-14活性在五个部分中定量：芽组织，芽呼吸，“根”组织（=根加真菌），“根”呼吸（=地下释放的CO2）和土壤。在这五个组分中检测到的总C-14中，M-P植物比NILI-P植物多分配3.9%（收获1）至11.5%（收获4）到地下组分（“根”组织，土壤和“根”呼吸）。NM+P和M-P植物之间的差异仅为上述差异的一半（收获2和3时分别为3.1%和4.4%，相比之下，NM-P和M-P之间的差异分别为6.4%和7.4%）。对M-P和NM-P植物之间根/冠比差异的校正消除了仅在前三次收获时观察到的碳分配差异。没有证据表明，增加的“根”呼吸速率或根沉积是负责增加碳转移到地下的M-P植物。
The flow of carbon from plant to fungus in ectomycorrhizal associations has not been well quantified. The objective of this study was to use C-14 to quantify the increase in fixed carbon translocated below ground in ectomycorrhizal relative to non-mycorrhizal willow (Salix viminalis L. Bowles hybrid). Rooted cuttings were inoculated with Thelephora terrestris (Ehrh). Fr. or left non-mycorrhizal. Non-mycorrhizal plants were grown at the same (4 mg kg(-1) bicarbonate-extractable P) (NM-P) or at a higher (21 mg kg(-1)) soil P concentration (NM+P), one at which the non-mycorrhizal plants were similar in size to the mycorrhizal (M-P) plants. At 41, 51, 76 and 89 days after planting, the shoots were exposed to a pulse of (CO2)-C-14. Plants were harvested after a 202 h chase period. The C-14 activity was quantified in five fractions: shoot tissue, shoot respiration, 'root' tissue (=roots plus fungi), 'root' respiration (=CO2 released below ground) and soil. Of the total C-14 detected in these five fractions, M-P plants allocated from 3.9% (harvest 1) to 11.5% (harvest 4) more to the below-ground fractions ('root' tissue, soil and 'root' respiration), than did the NILI-P plants. Differences between NM+P and M-P plants were only half of those above (3.1% and 4.4%, at harvests 2 and 3, respectively, compared to 6.4%, and 7.4%, respectively for the difference between NM-P and M-P). Correction for differences in root/shoot ratio between M-P and NM-P plants eliminated the observed differences in carbon distribution only at the first three harvests. There was no evidence for increased 'root' respiration rates or rhizodeposition being responsible for the increased carbon diverted below ground by M-P plants.