The PHD Finger Protein MMD1/DUET Ensures the Progression of Male Meiotic Chromosome Condensation and Directly Regulates the Expression of the Condensin Gene CAP-D3
The PHD Finger Protein MMD1/DUET Ensures the Progression of Male Meiotic Chromosome Condensation and Directly Regulates the Expression of the Condensin Gene CAP-D3
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PHD Finger 蛋白 MMD1/DUET 确保雄性减数分裂染色体缩合的进展，并直接调节缩合蛋白基因 CAP-D3 的表达。
DOI：
10.1105/tpc.16.00040
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发表时间：
2016
期刊：
影响因子：
11.6
通讯作者：
Wang Yingxiang
中科院分区：
文献类型：
--
作者：
Wang Jun;Niu Baixiao;Huang Jiyue;Wang Hongkuan;Yang Xiaohui;Dong Aiwu;Makaroff Christopher;Ma Hong;Wang Yingxiang
染色体凝聚是由凝聚素复合物介导的过程，是细胞分裂过程中染色体正确分离所必需的。与快速有丝分裂染色体凝聚不同，减数分裂染色体凝聚发生在相对较长的前期I，并且由于与染色体轴形成和同源物相互作用的协调而异常复杂。调控减数分裂染色体从前期I到中期I凝聚进程的分子机制尚不清楚。在这里，我们表明，theArabidopsis thalianameiotic PHD-指蛋白MMD 1/DUET所需的逐步压实前期I染色体中期I二价体。MMD 1 PHD结构域是其在染色体浓缩中发挥功能所必需的，并与甲基化组蛋白尾部结合。转录组分析和qRT-PCR结果表明，在mmd 1性母细胞中，几个凝聚素基因的表达量显著降低。此外，MMD 1特异性结合到凝聚素亚基基因CAP-D3的启动子区以增强其表达。此外，cap-d3突变体表现出类似的染色体凝聚缺陷，揭示了MMD 1依赖的机制，调节减数分裂染色体凝聚，其功能部分通过促进凝聚素基因的表达。总之，这些发现提供了强有力的证据表明，组蛋白阅读器MMD 1/DUET定义了调节减数分裂前期I染色体浓缩进展的重要步骤。
Chromosome condensation, a process mediated by the condensin complex, is essential for proper chromosome segregation during cell division. Unlike rapid mitotic chromosome condensation, meiotic chromosome condensation occurs over a relatively long prophase I and is unusually complex due to the coordination with chromosome axis formation and homolog interaction. The molecular mechanisms that regulate meiotic chromosome condensation progression from prophase I to metaphase I are unclear. Here, we show that theArabidopsis thalianameiotic PHD-finger protein MMD1/DUET is required for progressive compaction of prophase I chromosomes to metaphase I bivalents. The MMD1 PHD domain is required for its function in chromosome condensation and binds to methylated histone tails. Transcriptome analysis and qRT-PCR showed that several condensin genes exhibit significantly reduced expression inmmd1meiocytes. Furthermore, MMD1 specifically binds to the promoter region of the condensin subunit geneCAP-D3to enhance its expression. Moreover,cap-d3mutants exhibit similar chromosome condensation defects, revealing an MMD1-dependent mechanism for regulating meiotic chromosome condensation, which functions in part by promoting condensin gene expression. Together, these discoveries provide strong evidence that the histone reader MMD1/DUET defines an important step for regulating the progression of meiotic prophase I chromosome condensation.