Network signatures of nuclear and cytoplasmic density alterations in a model of pre and postmetastatic colorectal cancer
Network signatures of nuclear and cytoplasmic density alterations in a model of pre and postmetastatic colorectal cancer
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DOI：
10.1117/1.jbo.19.1.016016
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发表时间：
2014-01-01
影响因子：
3.5
通讯作者：
Phillips, Kevin G.
中科院分区：
文献类型：
--
作者：
Damania, Dhwanil;Subramanian, Hariharan;Phillips, Kevin G.
导致癌症发病的细胞具有细胞质和核结构改变，伴随着它们的异常遗传、表观遗传和分子扰动。虽然它是已知的，在原发性和转移性肿瘤细胞的结构变化可以通过在纳米和微米空间尺度上的细胞密度的变化进行量化，核和细胞质密度之间的相互依赖的关系作为致瘤潜力的函数尚未得到彻底的研究。我们提出了一种组合的光学方法，利用定量相位显微镜和分波光谱显微镜进行并行的细胞结构的结构表征。使用同基因SW480和SW620细胞系作为模型的前和转移后的结肠直肠癌的过渡，我们证明，核和细胞质纳米级的障碍，微米级干质量含量，平均干质量密度，和形状指标的干质量密度直方图是唯一相关的内部和跨不同的细胞室为一个给定的细胞类型。这些物理参数的相关性可以被解释为网络的节点的重要性和连接的独立性水平根据疾病阶段而不同。这项工作展示了光学衍生的生物物理参数如何在转移表型的架构编排期间在不同的细胞隔室内和跨不同的细胞隔室相关联。(C)2014年，美国光电仪器工程师学会（SPIE）
Cells contributing to the pathogenesis of cancer possess cytoplasmic and nuclear structural alterations that accompany their aberrant genetic, epigenetic, and molecular perturbations. Although it is known that architectural changes in primary and metastatic tumor cells can be quantified through variations in cellular density at the nanometer and micrometer spatial scales, the interdependent relationships among nuclear and cytoplasmic density as a function of tumorigenic potential has not been thoroughly investigated. We present a combined optical approach utilizing quantitative phase microscopy and partial wave spectroscopic microscopy to perform parallel structural characterizations of cellular architecture. Using the isogenic SW480 and SW620 cell lines as a model of pre and postmetastatic transition in colorectal cancer, we demonstrate that nuclear and cytoplasmic nanoscale disorder, micron-scale dry mass content, mean dry mass density, and shape metrics of the dry mass density histogram are uniquely correlated within and across different cellular compartments for a given cell type. The correlations of these physical parameters can be interpreted as networks whose nodal importance and level of connection independence differ according to disease stage. This work demonstrates how optically derived biophysical parameters are linked within and across different cellular compartments during the architectural orchestration of the metastatic phenotype. (C) 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)