Matrix-assisted laser desorption using a fast-atom bombardment ion source and a magnetic mass spectrometer.
Matrix-assisted laser desorption using a fast-atom bombardment ion source and a magnetic mass spectrometer.
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使用快原子轰击离子源和磁质谱仪进行基质辅助激光解吸。
DOI：
10.1002/rcm.1290060415
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Biemann,K
中科院分区：
文献类型：
--
作者：
Annan,RS;Kochling,HJ;Hill,JA;Biemann,K
使用常规快原子轰击（FAB）离子源在高质量双聚焦磁质谱仪中实现基质辅助激光解吸（MALD）。用XeF准分子激光器（353 nm）或氮激光器（337 nm）照射样品和基质（2，5-二羟基苯甲酸）的混合物产生的脉冲离子信号用焦平面检测器记录。分辨率（半峰全宽）为4500 atm/z1347.7（肽物质P），CsI簇离子为2500 atm/z10005.7，小蛋白细胞色素c（马）[M+H]+=m/z12360（平均值）的同位素簇为1250。用11 fmol P物质证明了灵敏度。进行测量扫描以产生更好的信噪比。除了更高的灵敏度和更低的基质干扰外，MALD相对于FAB的优势在于前者对盐的存在的敏感性更低，以及混合物中疏水性和亲水性组分之间的竞争。这一特征通过抹香鲸脱辅基肌红蛋白粗品部分胰蛋白酶消化物的完整MALD光谱得到证明，该蛋白质含有24种肽，代表了该蛋白质的整个序列。
A conventional fast‐atom bombardment (FAB) ion source was used to achieve matrix‐assisted laser desorption (MALD) in a high‐mass, double‐focusing, magnetic mass spectrometer. The pulsed ion signals generated by irradiation of a mixture of sample and matrix (2,5‐dihydroxybenzoic acid) with either a XeF excimer Laser (353 nm) or a nitrogen laser (337 nm) were recorded with a focal‐plane detector. A resolution (full‐width at half maximum) of 4500 was achieved atm/z1347.7 (the peptide substance P), 2500 for CsI cluster ions atm/z10005.7, and 1250 for the isotope cluster of the small protein cytochrome c (horse) [M+H]+=m/z12360 (average). Sensitivity is demonstrated with 11 fmol of substance P. A survey scan is taken to prodluce a better signal‐to‐noise ratio. In addition to higher sensitivity and lower matrix interference, lthe advantage of MALD over FAB is the former's lower susceptibility to the presence of salts, and competition between hydrophobic and bydrophilic components of a mixture. This feature is demonstrated by the complete MALD spectrum of a crude partial tryptic digest of spedrm‐whale apomyoglobin, containing 24 peptides, representing the entire sequence of this protein.