Measurement of cortical and hippocampal epileptiform activity in freely moving rats by means of implantable radiotelemetry
Measurement of cortical and hippocampal epileptiform activity in freely moving rats by means of implantable radiotelemetry
复制标题
DOI：
10.1016/j.jneumeth.2004.03.004
复制
发表时间：
2004-09-30
影响因子：
3
通讯作者：
Watson, WP
中科院分区：
文献类型：
--
作者：
Bastlund, JF;Jennum, P;Watson, WP
植入式无线电遥测技术已被用于测量皮层脑电图（EEG）、运动活动、体温和心血管参数。该技术允许从自由移动的动物中获取高质量的数据，而没有外部设备的复杂性。本文着重于用皮层脑电图、海马（HC）脑电图和肌电图（EMG）同步监测大鼠癫痫样活动的短期和长期方法。手术后监测温度昼夜节律（CRT），以估计动物术后恢复的需要。EMG电极的不同放置进行了评估，以尽量减少伪影和增加灵敏度。研究了急性注射40 mg/kg戊四唑（PTZ）或13.8 mg/kg红藻氨酸（KA）后癫痫样发作和发作间期活动的发生。在KA给药后5-8周还监测自发性癫痫发作的发生。本研究证明了一种敏感的方法，监测皮层脑电图，海马脑电图和肌电图的短期和长期植入式无线遥测在自由活动的大鼠。(C)2004 Elsevier B. V.保留所有权利。
Implanted radiotelemetry has been used for the measurement of cortical electroencephalogram (EEG), locomotor activity, body temperature and cardiovascular parameters. This technique allows high quality data acquisition from freely moving animals with no complications of externalised apparatus. This paper focuses on the methodology for short and long-term monitoring of epileptiform activity by simultaneous cortical EEG, hippocampal (HC) EEG and electromyogram (EMG) in rats. The circadian rhythm of temperature (CRT) was monitored after surgery to estimate the need for post surgical recovery of animals. Different placements of EMG electrodes were assessed in order to minimise artefacts and increase sensitivity. The occurrence of epileptiform ictal and interictal activity following an acute injection of either 40 mg/kg pentylenetetrazole (PTZ) or 13.8 mg/kg kainic acid (KA) was investigated. The occurrence of spontaneous seizures was also monitored 5-8 weeks after administration of KA. The present study demonstrated a sensitive method for monitoring cortical EEG, hippocampal EEG and EMG short and long-term by implantable radiotelemetry in freely moving rats. (C) 2004 Elsevier B.V. All rights reserved.