KGF Enhances Oral Epithelial Adhesion and Rete Peg Elongation via Integrins
KGF Enhances Oral Epithelial Adhesion and Rete Peg Elongation via Integrins
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KGF 通过整合素增强口腔上皮粘附和 Rete Peg 伸长
DOI：
10.1177/0022034517720360
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发表时间：
2017-12-01
影响因子：
7.6
通讯作者：
Zhao，Y. F.
中科院分区：
文献类型：
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作者：
Sa，G. L.;Xiong，X. P.;Zhao，Y. F.
口腔上皮粘附于固有层是口腔粘膜生理功能的基础，有助于抵抗细菌入侵、防止体液流失和维持日常咀嚼;因此，了解积极影响口腔上皮粘附的因素是一个非常有趣的研究课题。网钉通过扩大接触面积而有助于口腔上皮粘附，而整合素是介导上皮细胞粘附到基底膜的主要分子。角质细胞生长因子（KGF）可以促进皮肤中的网栓延长和各种细胞类型中整合素的表达。在此，我们测试的影响，粘膜下注射KGF在大鼠舌的腹侧表面口腔上皮粘附。这些数据证实，局部注射KGF提高了粘附力，延长了网钉，并增加了大鼠舌腹侧表面上整合素、KGF和KGF受体的丰度。然而，整合素拮抗剂HYD-1（Lys-Ile-Lys-Met-Val-Ile-Ser-Trp-Lys-Gly）可抑制KGF增强的上皮粘附和网栓延伸。此外，KGF促进整合素亚基α6，β4，α3和β1在人永生化口腔上皮细胞在二维和三维培养系统中的表达。体外细胞粘附实验表明，HYD-1抑制人永生化口腔上皮细胞在Matrigel上的粘附。值得注意的是，整合素，KGF和KGFR的表达在人类咀嚼粘膜与较长的网桩比衬里粘膜与较短的网桩更丰富。此外，网PEG长度与整合素、KGF和KGF受体的表达水平呈正相关。这些发现表明，KGF通过整合素增强口腔上皮粘附和网栓延伸。
Oral epithelial adhesion to the lamina propria underlies the physiologic function of the oral mucosa and contributes to resisting bacterial invasion, preventing body fluid loss, and maintaining routine chewing; thus, understanding the factors that positively influence oral epithelial adhesion is a research topic of great interest. Rete pegs contribute to oral epithelial adhesion by enlarging the contact areas, whereas integrins are the major molecules that mediate epithelial cell adhesion to the basement membrane. Keratinocyte growth factor (KGF) can promote both rete peg elongation in the skin and the expression of integrins in various cell types. Herein, we tested the effects of submucosal injection of KGF in the ventral surfaces of rat tongues on oral epithelial adhesion. The data confirmed that topical injection of KGF elevated the adhesive forces, elongated the rete pegs, and increased the abundance of integrins, KGF, and KGF receptor on the rat tongue ventral surface. However, HYD-1 (Lys-Ile-Lys-Met-Val-Ile-Ser-Trp-Lys-Gly), an integrin antagonist, inhibited the KGF-enhanced epithelial adhesion and rete peg elongation. Moreover, KGF promoted the expression of integrin subunits α6, β4, α3, and β1 in human immortalized oral epithelial cells in 2- and 3-dimensional culture systems. In vitro cell attachment assays demonstrated that HYD-1 inhibited the adhesion of human immortalized oral epithelial cells on Matrigel. Strikingly, the expression of integrins, KGF, and KGFR in human masticatory mucosae with longer rete pegs was more abundant than that in the lining mucosae with shorter rete pegs. In addition, rete peg lengths were positively correlated with the expression levels of integrins, KGF, and KGF receptor. These findings indicate that KGF strengthens oral epithelial adhesion and rete peg elongation via integrins.