INHIBITION OF MYELOID DIFFERENTIATION BY THE HELIX-LOOP-HELIX PROTEIN ID
INHIBITION OF MYELOID DIFFERENTIATION BY THE HELIX-LOOP-HELIX PROTEIN ID
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DOI：
10.1126/science.1372755
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发表时间：
1992-03-27
期刊：
影响因子：
56.9
通讯作者：
KADESCH, T
中科院分区：
文献类型：
--
作者：
KREIDER, BL;BENEZRA, R;KADESCH, T
Id是一种螺旋-环-螺旋（helix-loop-helix，HLH）蛋白，其抑制参与细胞类型特异性转录和细胞谱系定型的几种碱性螺旋-环-螺旋（basic helix-loop-helix，bHLH）蛋白的活性。髓样前体细胞系32 DC 13（G）表达Id信使RNA，当用粒细胞集落刺激因子诱导细胞终末分化时，Id信使RNA瞬时减少。伴随着Id信使RNA的减少，在核提取物中出现了DNA结合蛋白，其识别了典型的E盒基序，一些bHLH蛋白的DNA结合位点。Id互补DNA在32 DC 13（G）细胞中的组成型表达阻断了它们的分化能力和诱导E-box结合活性。这些结果表明，Id，因此，bHLH蛋白的功能在骨髓分化的过程中。
Id is a helix-loop-helix (HLH) protein that represses activity of several basic helix-loop-helix (bHLH) proteins involved in cell type-specific transcription and cell lineage commitment. The myeloid precursor cell line 32DC13(G) expressed Id messenger RNA, which was transiently decreased when cells were induced to terminally differentiate with granulocyte-colony-stimulating factor. Concomitant with the decrease of Id messenger RNA was the appearance in nuclear extracts of DNA binding proteins that recognized a canonical E-box motif, a DNA binding site for some bHLH proteins. Constitutive expression of an Id complementary DNA in 32DC13(G) cells blocked their ability to differentiate and to induce E-box-binding activity. These results suggest that Id and, hence, bHLH proteins function in the process of myeloid differentiation.