Synthesis, Stability, Cellular Uptake, and Blood Circulation Time of Carboxymethyl-Inulin Coated Magnetic Nanoparticles.
Synthesis, Stability, Cellular Uptake, and Blood Circulation Time of Carboxymethyl-Inulin Coated Magnetic Nanoparticles.
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DOI：
10.1039/c3tb20256a
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发表时间：
2013-06-14
期刊：
影响因子：
--
通讯作者：
Rinaldi C
中科院分区：
文献类型：
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作者：
Santiago-Rodríguez L;Lafontaine MM;Castro C;Méndez-Vega J;Latorre-Esteves M;Juan EJ;Mora E;Torres-Lugo M;Rinaldi C
通过在菊粉结构中引入羧基并与接枝有3-氨丙基三乙氧基硅烷的氧化铁纳米颗粒表面上的胺基偶联，用生物相容性、生物可降解性、非免疫原性的多糖菊粉包覆氧化铁纳米颗粒。所得的纳米粒子的特征在于通过FT-IR光谱，透射电子显微镜，动态光散射，zeta电位，SQUID磁强计，并相对于它们的能量耗散率在施加的交变磁场。纳米颗粒具有在70 ± 10 nm范围内的流体动力学直径，并且是超顺磁性的，对于233 kHz的施加场频率和20 - 48 kA/m范围内的施加场振幅，能量耗散率在58 - 175 W/g范围内。纳米颗粒在23 ° C至53 ° C的pH值范围内以及在水、PBS和培养基中短期储存时保持稳定。所述颗粒对永生化人癌细胞系Hey A8 FDR、A2780、MDA 468、MCF-7和Caco-2无细胞毒性。纳米颗粒很容易被Caco-2细胞以时间和浓度依赖的方式吸收，并被发现具有47 ± 3 min的药代动力学时间常数。颗粒的小尺寸，无细胞毒性和有效的能量耗散使其可用于生物医学应用，如磁流体热疗。
Iron oxide nanoparticles were coated with the biocompatible, biodegradable, non-immunogenic polysaccharide inulin by introduction of carboxyl groups into the inulin structure and conjugation with amine groups on the surface of iron oxide nanoparticles grafted with 3-aminopropyltriethoxysilane. The resulting nanoparticles were characterized by FT-IR spectroscopy, transmission electron microscopy, dynamic light scattering, zeta potential, SQUID magnetometry, and with respect to their energy dissipation rate in applied alternating magnetic fields. The nanoparticles had a hydrodynamic diameter in the range of 70 ± 10 nm and were superparamagnetic, with energy dissipation rates in the range of 58–175 W/g for an applied field frequency of 233 kHz and an applied field amplitude in the range of 20–48 kA/m. The nanoparticles were stable in a range of pH, at temperatures between 23°C and 53°C, and in short term storage in water, PBS, and culture media. The particles were non-cytotoxic to the immortalized human cancer cell lines Hey A8 FDR, A2780, MDA 468, MCF-7 and Caco-2. The nanoparticles were readily taken up by Caco-2 cells in a time and concentration dependent fashion, and were found to have a pharmacokinetic time constant of 47 ± 3 min. The small size, non-cytotoxicity, and efficient energy dissipation of the particles could make them useful for biomedical applications such as magnetic fluid hyperthermia.