The linker between LOV2-Ja and STK plays an essential role in the kinase activation by blue light in Arabidopsis phototropin1, a plant blue light receptor.
The linker between LOV2-Ja and STK plays an essential role in the kinase activation by blue light in Arabidopsis phototropin1, a plant blue light receptor.
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LOV2-Ja 和 STK 之间的连接子在拟南芥趋光素1（一种植物蓝光受体）中蓝光激活激酶中发挥着重要作用。
DOI：
10.1002/1873-3468.12028
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
S.
中科院分区：
文献类型：
--
作者：
Kashojiya;S.;Yoshihara;S.;Okajima;K. and Tokuotmi;S.
光致蛋白（Phototropin, phot）是植物中的一种蓝光受体，由LOV1、LOV2和丝氨酸/苏氨酸激酶（STK）几个结构域组成。LOV2是STK光激活的主要调控因子，但其具体机制尚不清楚。在这篇报道中，我们重点研究了LOV2和STK之间的连接区域，不包括Jα‐螺旋。对arabidopsisphot1 LOV2‐STK连接子区域取代基的光谱分析和激酶分析表明，该连接子参与了STK的激活。连接子中间的一个假定模块对STK的分子内信号传导和/或调控至关重要。
Phototropin (phot), a blue light receptor in plants, is composed of several domains: LOV1, LOV2, and a serine/threonine kinase (STK). LOV2 is the main regulator of light activation of STK. However, the detailed mechanism remains unclear. In this report, we focused on the linker region between LOV2 and STK excluding the Jα‐helix. Spectroscopy and a kinase assay for the substituents in the linker region ofArabidopsisphot1 LOV2‐STK indicated that the linker is involved in the activation of STK. A putative module in the middle of the linker would be critical for intramolecular signaling and/or regulation of STK.