Preparation of Human Olfactory Epithelial Biopsies for Downstream Analysis.
Preparation of Human Olfactory Epithelial Biopsies for Downstream Analysis.
复制标题
制备用于下游分析的人类嗅觉上皮活检。
DOI：
10.1007/978-1-0716-3425-7_10
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Goldstein,BradleyJ
中科院分区：
文献类型：
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作者：
Choi,Rhea;Ko,Tiffany;Finlay,JohnB;Hachem,RalphAbi;Jang,David;Goldstein,BradleyJ
嗅觉粘膜排列在鼻腔的一部分，容纳着负责气味转导的初级嗅觉感觉神经元以及支持细胞群。动物模型，特别是小鼠的外周嗅觉系统的研究取得了巨大的进步。然而，获得性人类嗅觉障碍缺乏有效的治疗方法，其中许多疾病涉及嗅黏膜的病理。因此，从嗅觉障碍受试者或对照中获取人类嗅觉活检样本的能力可能是有价值的。在这里，我们描述了从受试者身上收集嗅觉组织并为下游分析准备样本的既定技术，如免疫组织化学、流式细胞术、单细胞RNA测序或染色质研究。
The olfactory mucosa, lining a portion of the nasal cavity, houses the primary olfactory sensory neurons responsible for odor transduction, along with supporting cell populations. Tremendous advances have come from studying the peripheral olfactory system in animal models, especially the mouse. However, acquired human olfactory disorders lack effective therapies, and many of these conditions involve pathology in the olfactory mucosa. Thus, the ability to obtain human olfactory biopsy samples from subjects with olfactory dysfunction, or controls, may be of value. Here, we describe established techniques for collecting olfactory tissue from human subjects and preparing samples for downstream assays such as immunohistochemistry, flow cytometry, single-cell RNA-sequencing, or chromatin studies.