Salt sensitivity in chickpea (Cicer arietinumL.): ions in reproductive tissues and yield components in contrasting genotypes
Salt sensitivity in chickpea (Cicer arietinumL.): ions in reproductive tissues and yield components in contrasting genotypes
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DOI：
10.1111/pce.12506
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发表时间：
2015-08-01
影响因子：
7.3
通讯作者：
Colmer, Timothy D.
中科院分区：
文献类型：
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作者：
Kotula, Lukasz;Khan, Hammad A.;Colmer, Timothy D.
鹰嘴豆（Cicer arietinumL.）的繁殖阶段受盐度影响，但对其潜在原因知之甚少。我们研究了生殖结构中高浓度的Na+和Cl-是否会影响生殖过程。在盆栽土壤中施用0、35、50mm NaCl，鹰嘴豆基因型的耐受性不同。评价了开花和败育、荚果数、空荚率、种子数和大小。测定了不同植物组织中Na+、K+和Cl-的浓度，并利用x射线微量分析测定了发育中的生殖结构特定细胞中的Na+、K+和Cl-的浓度。生理盐水条件下，以种子产量衡量的生殖成功的基因型差异与更好地维持开花和更高的荚果数量（从而增加种子数量）有关，而所有基因型的种子大小都减少了。尽管繁殖成功率存在差异，但耐受性基因型（Genesis836）和敏感性基因型（Rupali）在发育豆荚的早期生殖组织中Na+和Cl-的积累并没有差异。同样，耐盐性与繁殖时叶片或成熟时种子中盐离子的积累无关。我们研究了盐度对鹰嘴豆生殖过程的不利影响是否与生殖组织中的离子积累有关。采用x射线微量定量分析方法，对两种耐盐基因型生殖器官发育中特定细胞的Na、K和Cl浓度进行了测定。尽管盐碱地植物的种子产量存在较大的基因型差异，但两种基因型植物胚珠发育和荚果壁中的Na、K和Cl浓度相似。虽然这些早期生殖组织的钠浓度有所增加，但浓度相对较低。Na在成熟种子中也有所增加，但这并不影响它们随后的发芽。
The reproductive phase in chickpea (Cicer arietinumL.) is affected by salinity, but little is known about the underlying cause. We investigated whether high concentrations of Na+ and Cl- in the reproductive structures influence reproductive processes. Chickpea genotypes contrasting in tolerance were subjected to 0, 35 or 50mm NaCl applied to soil in pots. Flower production and abortion, pod number, percentage of empty pods, seed number and size were evaluated. The concentrations of Na+, K+ and Cl- were measured in various plant tissues and, using X-ray microanalysis, in specific cells of developing reproductive structures. Genotypic variation in reproductive success measured as seed yield in saline conditions was associated with better maintenance of flower production and higher numbers of filled pods (and thus seed number), whereas seed size decreased in all genotypes. Despite the variation in reproductive success, the accumulation of Na+ and Cl- in the early reproductive tissues of developing pods did not differ between a tolerant (Genesis836) and a sensitive (Rupali) genotype. Similarly, salinity tolerance was not associated with the accumulation of salt ions in leaves at the time of reproduction or in seeds at maturity.We studied whether the adverse effects of salinity on reproductive processes in chickpea are associated with ion accumulation in reproductive tissues. Quantitative X-ray microanalysis was used to evaluate the concentrations of Na, K and Cl in specific cells of developing reproductive organs of two genotypes of contrasting salt tolerance. Despite large genotypic differences for seed yield of plants in saline soil, Na, K and Cl concentrations in developing ovules and pod walls were similar for the two genotypes. Although Na concentrations had increased in these early reproductive tissues the concentrations were relatively low. Na had also increased in mature seeds, but this did not impact their subsequent germination.