The arabidopsis dynamin-like proteins ADL1C and ADU1E play a critical role in mitochondrial morphogenesis
The arabidopsis dynamin-like proteins ADL1C and ADU1E play a critical role in mitochondrial morphogenesis
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DOI：
10.1105/tpc.015222
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发表时间：
2003-10-01
期刊：
影响因子：
11.6
通讯作者：
Hwang, IW
中科院分区：
文献类型：
--
作者：
Jin, JB;Bae, HJ;Hwang, IW
动力蛋白相关蛋白是高分子量GTP结合蛋白，参与多种生物过程。本文报道了拟南芥中两个动力蛋白同源物的功能特征，即拟南芥动力蛋白样1C （ADL1C）和拟南芥动力蛋白样1E （ADL1E）。ADL1C和ADL1E与动力蛋白家族成员的氨基酸序列高度相似。然而，这两种蛋白都缺乏c端Pro-rich结构域和pleckstrin同源结构域。从叶细胞获得的原生质体中表达显性阴性突变体ADL1C[K48E]导致线粒体伸长异常。此外，ADL1E基因的T-DNA插入突变导致线粒体异常伸长，而原生质体中ADL1C和ADL1E的短暂表达挽救了线粒体异常伸长。在拟南芥原生质体的免疫组织化学和体内靶向实验中，ADL1C和ADL1E以大量斑点的形式出现，两种蛋白共定位。这些斑点部分与线粒体标记f1 - atp - γ:RFP和位于线粒体尖端的ADL2b共定位。这些结果提示ADL1C和ADL1E可能在植物细胞线粒体分裂中起关键作用。
Dynamin-related proteins are high molecular weight GTP binding proteins and have been implicated in various biological processes. Here, we report the functional characterization of two dynamin homologs in Arabidopsis, Arabidopsis dynamin-like 1C (ADL1C) and Arabidopsis dynamin-like 1E (ADL1E). ADL1C and ADL1E show a high degree of amino acid sequence similarity with members of the dynamin family. However, both proteins lack the C-terminal Pro-rich domain and the pleckstrin homology domain. Expression of the dominant-negative mutant ADL1C[K48E] in protoplasts obtained from leaf cells caused abnormal mitochondrial elongation. Also, a T-DNA insertion mutation at the ADL1E gene caused abnormal mitochondrial elongation that was rescued by the transient expression of ADL1C and ADL1E in protoplasts. In immunohistochemistry and in vivo targeting experiments in Arabidopsis protoplasts, ADL1C and ADL1E appeared as numerous speckles and the two proteins colocalized. These speckles were partially colocalized with F1-ATPase-gamma:RFP, a mitochondrial marker, and ADL2b localized at the tip of mitochondria. These results suggest that ADL1C and ADL1E may play a critical role in mitochondrial fission in plant cells.