Antibody response to an autogenous vaccine and serologic profile for Streptococcus suis capsular type 1/2.
Antibody response to an autogenous vaccine and serologic profile for Streptococcus suis capsular type 1/2.
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发表时间：
2002
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通讯作者：
L. Lapointe;S. D’Allaire;A. Lebrun;S. Lacouture;M. Gottschalk
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作者：
L. Lapointe;S. D’Allaire;A. Lebrun;S. Lacouture;M. Gottschalk
使用超声处理的细菌，用猪链球菌荚膜1/2型菌株开发了自体疫苗。本研究的目的是评价接种疫苗后的抗体应答，并评估显示S.在6- 8周龄猪中的猪囊1/2型感染。使用疫苗抗原的酶联免疫吸附试验被标准化。涉及2头对照猪和4头接种疫苗的4周龄猪的初步研究结果表明，所有接种疫苗的猪分别产生针对34和43 kDa 2种蛋白的抗体，4头接种疫苗的猪中有3头产生针对117 kDa溶菌酶释放蛋白的抗体。对于血清学特征，在2、4、6、8和10周龄时对来自感染猪群的30头猪进行血液采样。在第6 - 8周观察到最低抗体水平，推测与母体免疫力下降相对应。在10周龄时观察到显著增加，即在牛群中出现临床体征后不久。对于疫苗接种田间试验，将新断奶的1周龄仔猪分为2组，每组200头仔猪（对照组和疫苗接种组）;在12周内每隔2周从每组36头仔猪中采集血样。在接种后4周观察到抗体应答的显著增加（P < 0.05），并且抗体水平保持高水平直到实验结束。在对照组中，仅在13周龄时观察到增加，可能是对自然感染的反应。猪对疫苗的反应差异很大，部分归因于接种时母体抗体的水平。未发现S.在对照组或接种组中观察到猪流感，因此无法评估疫苗赋予的保护作用。
An autogenous vaccine was developed, using sonicated bacteria, with a strain of Streptococcus suis capsular type 1/2. The objectives of this study were to evaluate the antibody response following vaccination and to assess the changes in antibody levels in pigs from a herd showing clinical signs of S. suis capsular type 1/2 infection in 6- to 8-week-old pigs. An enzyme-linked immunosorbent assay using the vaccine antigen was standardized. Results from a preliminary study involving 2 control and 4 vaccinated 4-week-old pigs indicated that all vaccinated pigs produced antibodies against 2 proteins of 34 and 43 kDa, respectively, and, in 3 out of 4 vaccinated pigs, against the 117-kDa muramidase-released protein. For the serologic profile, groups of 30 pigs from the infected herd were blood sampled at 2, 4, 6, 8, and 10 weeks of age. The lowest antibody level was observed between weeks 6 and 8, presumably corresponding to a decrease in maternal immunity. A marked increase was seen at 10 weeks of age, shortly after the onset of clinical signs in the herd. For the vaccination field trial, newly weaned, one-week-old piglets were divided into 2 groups of 200 piglets each (control and vaccinated); blood samples were collected from 36 piglets in each group at 2-week intervals for 12 weeks. A significant increase (P < 0.05) in antibody response was observed 4 weeks following vaccination and the level of antibodies stayed high until the end of the experiment. In the control group, the increase was only observed at 13 weeks of age, probably in response to a natural infection. The response to the vaccine varied considerably among pigs and was attributed, in part, to the levels of maternal antibodies at the time of vaccination. No outbreak of S. suis was observed in the control or vaccinated groups, so the protection conferred by the vaccine could not be evaluated.