Immunohistochemical study of cytochrome P-45011β -hydroxylase in human adrenal cortex with mineralo- and glucocorticoid excess
Immunohistochemical study of cytochrome P-45011β -hydroxylase in human adrenal cortex with mineralo- and glucocorticoid excess
复制标题
盐皮质激素和糖皮质激素过量时人肾上腺皮质细胞色素 P-45011β-羟化酶的免疫组织化学研究
作者：
H. Sasano;M. Okamoto;N. Sasano
用抗牛肾上腺皮质线粒体P-45011β的特异性单抗，对人、猪和牛的肾上腺进行了细胞色素P-45011β羟化的免疫组织化学研究。结果表明，人、猪和牛的肾上腺中存在糖皮质激素和矿质激素的过量。P-45011β在牛、猪和人正常肾上腺的三个皮质区均有表达，尤以束带(ZF)和网状带(ZR)最为明显。P-45011β在库欣病的皮质微结节及内侧ZF和ZR表达较强，而在特发性醛固酮增多症患者的球状带和外侧ZF表达较强，与类固醇激素生成活跃部位相对应。在库欣综合征和原发性醛固酮增多症的腺瘤中，致密细胞通常染色良好。在与腺瘤相连的肾上腺中，免疫反应仅见于ZG细胞，而在ZF和ZR细胞中未见。
Cytochrome P-450 specific for steroid 11β-hydroxylation (P-45011β ) was immunohistochemically demonstrated in the adrenal glands of human, pig and bovine and of mineralo- and glucocorticoid excess using a specific monoclonal antibody against P-45011β of bovine adrenocortical mitochondria. P-45011β was present in all three cortical zones of the histologically normal adrenal glands of bovine, pig and human, particularly in the zona fasciculata (ZF) and reticularis (ZR). The P-45011β immunoreactivity was intensive in cortical micronodules and inner ZF and ZR in Cushing's disease, and relatively intensive in the zona glomerulosa (ZG) and outer ZF in idiopathic hyperaldosteronism (IHA), corresponding to the sites of active steroidogenesis. In adenomas with Cushing's syndrome and primary aldosteronism, compact cells were generally stained well. In the adrenal glands attached to the adenomas, immunoreactivity was observed only focally in ZG cells but not in ZF and ZR cells.
在牛肾上腺皮质细胞的原代培养物中促肾上腺皮质激素诱导 11 β-羟化酶。
DOI：
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发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Kramer,RE;Simpson,ER;Waterman,MR
通讯作者：
Waterman,MR
DOI：
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发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Yanagibashi,K;Haniu,M;Shively,JE;Shen,WH;Hall,P
通讯作者：
Hall,P