Determination of a cleavage site of presenilin 2 protein in stably transfected SH-SY5Y human neuroblastoma cell lines
Determination of a cleavage site of presenilin 2 protein in stably transfected SH-SY5Y human neuroblastoma cell lines
复制标题
DOI：
10.1006/bbrc.1997.7730
复制
发表时间：
1997-11-26
影响因子：
3.1
通讯作者：
Tabira, T
中科院分区：
文献类型：
--
作者：
Shirotani, K;Takahashi, K;Tabira, T
早老素1（PS1）和早老素2（PS2）基因的突变与早发性常染色体显性遗传家族性阿尔茨海默病相关，基因产物经内蛋白水解加工产生N-末端片段（NTF）和C-末端片段（CTF）。我们研究了PS2蛋白在稳定转染的人神经母细胞瘤细胞中的切割位点。通过阴离子交换层析和亲和层析相结合的方法分离得到23 kD的PS2-CTF，并直接测序。PS2-CTF的N-末端起始于残基307，这表明在大亲水环的近端部分中在Lys（306）和Leu（307）之间发生切割。该位点接近在PS1蛋白中观察到的切割位置。(C)1997年学术出版社。
Mutations in the presenilin 1 (PS1) and presenilin 2 (PS2) genes are associated with early-onset autosomal dominant familial Alzheimer's disease, and the gene products are endoproteolytically processed to yield N-terminal fragments (NTF) and C-terminal fragments (CTF). We have studied the cleavage site of the PS2 protein in stably transfected human neuroblastoma cells. The 23 kD PS2-CTF was isolated by a combination of anion exchange chromatography and affinity chromatography and directly sequenced. The N-terminus of the PS2-CTF started at residue 307, which indicated that the cleavage occurs between Lys(306) and Leu(307) in the proximal portion of the large hydrophilic loop. This site is close to the cleavage positions observed in the PS1 protein. (C) 1997 Academic Press.