The use of the API 20 NE bacteria classification procedure to identify Pasteurellaceae strains in rodents and rabbits.
The use of the API 20 NE bacteria classification procedure to identify Pasteurellaceae strains in rodents and rabbits.
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发表时间：
2004
影响因子：
0.8
通讯作者：
R. Boot;M. Brink;P. Handgraaf;R. Timmermans
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作者：
R. Boot;M. Brink;P. Handgraaf;R. Timmermans
40株不依赖生长因子的巴氏杆菌科菌株代表了啮齿动物和兔子中最知名的分类群，由四个实验动物诊断实验室使用其常规方法对API 20 NE进行了分析。在被归类为巴氏杆菌科的细菌菌株数量上发现了显著差异。四个实验室的综合结果显示，对40种菌株进行的160次检测中，有136次（85%）得出了该科的分类结果。来自API 20 NE数据库中的种（产气巴斯德菌、多杀巴斯德菌、嗜肺巴斯德菌）的23种巴斯德菌科菌株（API分类群菌株）和来自未包括的分类群的17种菌株（非API分类群菌株）被分类为具有相似频率的科。实验室之间的巴氏杆菌种属命名存在显著差异，40株菌株中只有9株（22.5%）在种属上完全一致。在对多杀性巴氏杆菌和嗜肺巴氏杆菌进行的检测中，分别有42%和52%导致错误分类。相反，38%的非API分类群菌株的图谱被鉴定为多杀性巴氏杆菌或嗜肺巴氏杆菌。我们得出结论，通过API 20 NE系统在种水平上鉴定巴氏杆菌科是不可靠的，但该系统可用于将来自啮齿动物和兔的各种巴氏杆菌科分类为细菌科。
Forty growth-factor independent Pasteurellaceae strains representing most known taxa from rodents and rabbits were API 20 NE profiled by four laboratory animal diagnostic laboratories using their routine methodology. Significant differences were found in the number of bacterial strains classified with the Pasteurellaceae . The results of the four laboratories taken together showed that 136 (85 %) of the 160 tests carried out with the 40 strains led to classification with the family. The 23 Pasteurellaceae strains from species (Pasteurella aerogenes, P. multocida, P. pneumotropica) present in the API 20 NE data base (API taxa strains) and the 17 strains from taxa not included (non - API taxa strains) were classified with the family with similar frequency. Pasteurella species designation differed significantly between the laboratories and full agreement in speciation was found with only 9 (22.5 %) of the 40 strains. Of the tests carried out with P. multocida and P. pneumotropica, 42 and 52 % respectively led to misclassification. Conversely 38 % of the profiles obtained with non- API taxa strains led to identification as P. multocida or P. pneumotropica. We conclude that identification of Pasteurellaceae to the species level by the API 20 NE system is unreliable, but the system is useful to classify various Pasteurellaceae taxa from rodents and rabbits with the bacterial family.Â