Visualization of lncRNA and mRNA Structure Models Within the Integrative Genomics Viewer.
Visualization of lncRNA and mRNA Structure Models Within the Integrative Genomics Viewer.
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综合基因组学查看器中 lncRNA 和 mRNA 结构模型的可视化。
DOI：
10.1007/978-1-0716-1158-6_2
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Weeks KM
中科院分区：
文献类型：
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作者：
Busan S;Weeks KM
每一类RNA都形成影响生物功能的碱基配对结构。RNA结构的化学探测，特别是随着SHAPE-MaP等策略的出现，极大地扩展了RNA结构检测的规模和定量准确性。这些方法已经能够对mRNA和lncRNA进行大规模的结构研究，但是这些RNA的长度和复杂性使得数据的解释具有挑战性。我们已经通过开源的Integrative Genomics Viewer（IGV）创建了可用的模块，用于直接可视化RNA结构以及相关和互补的实验数据。在这里，我们提出了详细的和逐步的策略，探索和可视化复杂的RNA结构IGV。个人可以使用这些说明和提供的样本数据，以便熟练地使用IGV来可视化RNA结构模型以及有用的相关信息。
Every class of RNA forms base-paired structures that impact biological functions. Chemical probing of RNA structure, especially with the advent of strategies such as SHAPE-MaP, vastly expands the scale and quantitative accuracy over which RNA structure can be examined. These methods have enabled large-scale structural studies of mRNAs and lncRNAs, but the length and complexity of these RNAs makes interpretation of the data challenging. We have created modules available through the open-source Integrative Genomics Viewer (IGV) for straightforward visualization of RNA structures and associated and complementary experimental data. Here we present detailed and stepwise strategies for exploring and visualizing complex RNA structures in IGV. Individuals can use these instructions and supplied sample data to become adept at using IGV to visualize RNA structure models in conjunction with useful allied information.