Thermosensitive and photocrosslinkable hydroxypropyl chitin-based hydrogels for biomedical applications
Thermosensitive and photocrosslinkable hydroxypropyl chitin-based hydrogels for biomedical applications
复制标题
用于生物医学应用的热敏和光交联羟丙基甲壳素水凝胶
DOI：
10.1016/j.carbpol.2018.03.031
复制
发表时间：
2018
影响因子：
11.2
通讯作者：
Jiang Xulin
中科院分区：
文献类型：
--
作者：
Yuan Meng;Bi Bo;Huang Jiachang;Zhuo Renxi;Jiang Xulin
基于热敏聚合物的原位形成可注射水凝胶正在研究用于组织工程应用。然而，这类水凝胶的主要限制是低凝胶稳定性和生理条件下的弱机械性能。在这里，温敏性羟丙基甲壳素（HPCH）是均匀合成的，然后通过甲基丙烯酸缩水甘油酯与可光交联的甲基丙烯酸酯基团官能化，生成甲基丙烯酸缩水甘油酯改性的HPCH（GM-HPCH）。所获得的新的GM-HPCH聚合物在低浓度（2wt%的PBS中）下表现出类似的可逆热敏溶胶-凝胶转变行为。物理热凝胶化GM-HPCH水凝胶能够在生理条件下通过UV照射进行光交联，以形成增强的稳定的和机械强度强的水凝胶。通过控制甲基丙烯酸酯基团的取代度和紫外光照射时间可以调节水凝胶的力学性能、溶胀和降解行为。细胞毒性试验表明，光交联热凝胶无细胞毒性。可光交联的GM-HPCH热凝胶在生物医学领域具有巨大的应用潜力。
In situforming injectable hydrogels based on thermosensitive polymers are being investigated for tissue engineering applications. However, the major limitations of this kind of hydrogels are low gel stability and weak mechanical properties under physiological conditions. Here, thermosensitive hydroxypropyl chitin (HPCH) was synthesized homogenously and subsequently functionalized with photocrosslinkable methacrylate groupsviaglycidyl methacrylate to generate glycidyl methacrylate-modified HPCH (GM-HPCH). The obtained new GM-HPCH polymers exhibited similar reversible thermosensitive sol–gel transition behaviors at a low concentration (2 wt% in PBS). The physical thermogelation GM-HPCH hydrogels were able to be photocrosslinked by UV irradiation under physiological conditions to form enhanced stable and mechanically strong hydrogels. The mechanical property, swelling and degradation behavior of the hydrogels could be tuned by controlling the degree of substitution of methacrylate groups and UV exposure time. Cytotoxicity test displayed that the photocrosslinked thermogels were non-cytotoxic. The photocrosslinkable GM-HPCH thermogels hold great potential for biomedical applications.