Hierarchical polymeric scaffolds support the growth of MC3T3-E1 cells
Hierarchical polymeric scaffolds support the growth of MC3T3-E1 cells
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DOI：
10.1007/s10856-015-5453-z
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发表时间：
2015-02-01
影响因子：
3.7
通讯作者：
Yousefi, Azizeh-Mitra
中科院分区：
文献类型：
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作者：
Akbarzadeh, Rosa;Minton, Joshua A.;Yousefi, Azizeh-Mitra
组织工程利用生物学和工程学的原理来维持3D细胞生长并促进组织修复和/或再生。在这项研究中，宏观/微孔支架结构已开发使用混合固体自由成型制造/热诱导相分离（TIPS）技术。将聚（乳酸-共-乙醇酸）（PLGA）溶解在1，4-二氧六环中，通过TIPS方法制备微孔基质。三维生物绘图技术用于制造由聚乙二醇（PEG）制成的三维大孔结构。在TIPS过程之前将PEG构建体包埋在PLGA溶液内，随后在溶剂去除（1，4-二氧杂环己烷）之后提取PEG，产生大孔/微孔结构。这些具有双峰孔径分布（300 μ m）的分级支架含有由提取的PEG产生的正交互连的宏通道。通过调整3D生物芯片的分配参数来改变宏通道的直径。体外细胞培养21天的小鼠MC 3 T3-E1细胞系证明，这些支架可以提供一个有利的环境，以支持细胞粘附和生长。
Tissue engineering makes use of the principles of biology and engineering to sustain 3D cell growth and promote tissue repair and/or regeneration. In this study, macro/microporous scaffold architectures have been developed using a hybrid solid freeform fabrication/thermally induced phase separation (TIPS) technique. Poly(lactic-co-glycolic acid) (PLGA) dissolved in 1,4-dioxane was used to generate a microporous matrix by the TIPS method. The 3D-bioplotting technique was used to fabricate 3D macroporous constructs made of polyethylene glycol (PEG). Embedding the PEG constructs inside the PLGA solution prior to the TIPS process and subsequent extraction of PEG following solvent removal (1,4-dioaxane) resulted in a macro/microporous structure. These hierarchical scaffolds with a bimodal pore size distribution (300 mu m) contained orthogonally interconnected macro-channels generated by the extracted PEG. The diameter of the macro-channels was varied by tuning the dispensing parameters of the 3D bioplotter. The in vitro cell culture using murine MC3T3-E1 cell line for 21 days demonstrated that these scaffolds could provide a favorable environment to support cell adhesion and growth.