Optimization of Ex Vivo Machine Perfusion and Transplantation of Vascularized Composite Allografts.
Optimization of Ex Vivo Machine Perfusion and Transplantation of Vascularized Composite Allografts.
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DOI：
10.1016/j.jss.2021.09.005
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发表时间：
2022-03
期刊：
影响因子：
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通讯作者：
Uygun K
中科院分区：
文献类型：
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作者：
Burlage LC;Lellouch AG;Taveau CB;Tratnig-Frankl P;Pendexter CA;Randolph MA;Porte RJ;Lantieri LA;Tessier SN;Cetrulo CL Jr;Uygun K
机械灌流作为一种有效的血管复合异体移植(VCA)组织保存方法正受到人们的广泛关注。本研究的目的是开发一种用于啮齿动物后肢的体外亚常温充氧机灌流(SNMP)的方案，并在异位后肢移植模型中验证我们的方案。在这项优化研究中，我们比较了三种不同的解决方案在6小时的简单网络管理(n=4每组)。10条对照肢体保存在静态冷藏保存液中)。在SNMP期间，我们监测了动脉血流量、乳酸水平和水肿。实验结束后，取肌肉组织进行组织学检查和能荷分析。我们通过30天的随访(n=13)验证了异位肢体移植模型的最佳灌注方案。对照组移植未经处理的肢体(n=5)和后肢SCS保存6或24 h的肢体(n=4和n=5)。在SNMP期间，所有组的动脉流出量增加，乳酸清除量降低。HBOC-201组的总水肿率明显低于BSA组(p=0.005)，4.9%(4.3-6.1)比48.8%(39.1-53.2)，但与BSA+聚乙二醇组(p=0.19)无明显差异。与灌流无细胞氧气载体HBOC-201的肢体相比，SCS对照组的能量电荷水平降低了4倍，分别为0.10(0.07-0.17)和0.46(0.42-0.49)(p=0.002)。与传统的SCS相比，使用无细胞氧气载体HBOC-201对啮齿动物后肢进行6小时的体外SNMPs具有更好的组织保存效果。
Machine perfusion is gaining interest as an efficient method of tissue preservation of Vascularized Composite Allografts (VCA). The aim of this study was to develop a protocol for ex vivo subnormothermic oxygenated machine perfusion (SNMP) on rodent hindlimbs and to validate our protocol in a heterotopic hindlimb transplant model. In this optimization study we compared three different solutions during 6 hours of SNMP (n=4 per group). Ten control limbs were stored in a preservation solution on Static Cold Storage [SCS]). During SNMP we monitored arterial flowrate, lactate levels and edema. After SNMP, muscle biopsies were taken for histology examination and energy charge analysis. We validated the best perfusion protocol in a heterotopic limb transplantation model with 30-day follow up (n=13). As controls, we transplanted untreated limbs (n=5) and hindlimbs preserved with either 6 or 24 hours of SCS (n=4 and n=5). During SNMP, arterial outflow increased, and lactate clearance decreased in all groups. Total edema was significantly lower in the HBOC-201 group compared to the BSA group (p=0.005), 4.9 (4.3–6.1) vs. 48.8 (39.1–53.2) percentage, but not to the BSA + PEG group (p=0.19). Energy charge levels of SCS controls decreased 4-fold compared to limbs perfused with acellular oxygen carrier HBOC-201, 0.10 (0.07–0.17) vs. 0.46 (0.42–0.49) respectively (p=0.002). Six hours ex vivo SNMP of rodent hindlimbs using an acellular oxygen carrier HBOC-201 results in superior tissue preservation compared to conventional SCS.
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影响因子：
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作者：
Bejaoui M;Pantazi E;Calvo M;Folch-Puy E;Serafín A;Pasut G;Panisello A;Adam R;Roselló-Catafau J
通讯作者：
Roselló-Catafau J
DOI：
10.1111/ajt.12727
发表时间：
2014-06
期刊：
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons
影响因子：
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作者：
Bruinsma BG;Yeh H;Ozer S;Martins PN;Farmer A;Wu W;Saeidi N;Op den Dries S;Berendsen TA;Smith RN;Markmann JF;Porte RJ;Yarmush ML;Uygun K;Izamis ML
通讯作者：
Izamis ML
影响因子：
--
作者：
CONSOLI, A;NURJHAN, N;GERICH, JE
通讯作者：
GERICH, JE
DOI：
10.1016/s0967-2109(02)00070-4
发表时间：
2002-12-01
期刊：
CARDIOVASCULAR SURGERY
影响因子：
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作者：
Blaisdell, FW
通讯作者：
Blaisdell, FW
影响因子：
2.2
作者：
Mueller, Sabine;Constantinescu, Mihai A.;Voegelin, Esther
通讯作者：
Voegelin, Esther