SERUM LACTIC DEHYDROGENASE ACTIVITY: AN ANALYTICAL ASSESSMENT OF CURRENT ASSAYS.
SERUM LACTIC DEHYDROGENASE ACTIVITY: AN ANALYTICAL ASSESSMENT OF CURRENT ASSAYS.
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血清乳酸脱氢酶活性：当前检测的分析评估。
DOI：
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发表时间：
1963
影响因子：
9.3
通讯作者：
Warren E. C. Wacker
中科院分区：
文献类型：
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作者：
E. Amador;L. E. Dorfman;Warren E. C. Wacker
通过“正向”（乳酸+ DPN 至丙酮酸+ DPNH + H）法分光光度法测定血清LDH 活性，反应速率呈线性，活性与添加的血清量成正比，重现性为1.3%。试剂混合物可冷冻保存 6 个月，冻干状态可无限期保存。正常与异常血清 LDH 活性的分离不良是由于非线性校准曲线和“向后”分光光度测定的反应速率降低造成的。二硝基苯肼比色测定被认为不适合临床使用，因为试剂校准曲线的微小变化会导致活性的较大变化，并且因为与“正向”分光光度测定获得的活性相比，比色活性不准确。
Serum LDH activities assayed spectrophotometrically by the "forward" (lactate + DPN to pyruvate + DPNH + H) method have linear reaction rates, activity proportional to the amount of serum added, and a reproducibility of 1.3%. The reagent mixture can be stored frozen for 6 months and in the lyophilized state indefinitely. Poor separation of normal from abnormal serum LDH activities results from the nonlinear calibration curves and decreasing reaction rates of the "backward" spectro-photometric assay. The dinitrophenylhydrazine colorimetric assay is deemed unsuitable for clinical use because large variations in activity result from minor variations in the reagent calibration curve, and because colorimetric activities are inaccurate as compared to activities obtained with the "forward" spectrophotometric assay.