The Constipation-Relieving Property of d-Tagatose by Modulating the Composition of Gut Microbiota
The Constipation-Relieving Property of d-Tagatose by Modulating the Composition of Gut Microbiota
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DOI：
10.3390/ijms20225721
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发表时间：
2019-11-01
影响因子：
5.6
通讯作者：
Wang, Hong
中科院分区：
文献类型：
--
作者：
Liang, Yu-Xuan;Wen, Peng;Wang, Hong
据报道，D-塔格糖是一种单糖，也是一种膳食补充剂，在食品工业中具有广泛的适用性，然而，其益生元活性、抗便秘作用和相关机制仍不清楚。本研究以洛哌丁胺诱导的昆明小鼠便秘为动物模型，通过胃肠传输实验和排便实验，评价d-塔格糖对便秘的预防作用。此外，通过评价不同小鼠组间生化指标的变化和肠道菌群16 S rRNA扩增产物的分析，阐明了其潜在的机制。结果表明，d-塔格糖给药后6小时内的胃肠通过率、粪便数和体重均显著增加。此外，d-塔格糖显著提高血清乙酰胆碱（Ach）和P物质（SP）水平，而血清一氧化氮（NO）水平显著降低。此外，16 S rRNA测序分析显示，由便秘引起的肠道微生物群的变化通过d-塔格糖治疗得以恢复。综上所述，本研究表明，d-塔格糖作为膳食补充剂给药可以有效地预防和缓解昆明小鼠便秘，并且它是一种有希望的具有缓解便秘特性的益生元候选物。
d-tagatose, a monosaccharide as well as a dietary supplement, has been reported as having a wide range of applicability in the food industry, however, the prebiotic activity, anticonstipation effects, and related mechanisms are still unclear. In this study, using the loperamide-induced constipation Kunming mice as the animal model, the effects of d-tagatose for the prevention of constipation were evaluated by gastrointestinal transit experiment and defecation experiment. Furthermore, the underlying mechanism was clarified by evaluating the change of the biochemical indicators and analyzing 16S rRNA amplicon of gut microbiota among the different mice groups. The results showed that the gastrointestinal transit rate, fecal number, and weight in six hours were significantly enhanced after the administration of d-tagatose. In addition, d-tagatose significantly increased the serum levels of acetylcholine (Ach) and substance P (SP), whereas the serum levels of nitric oxide (NO) were significantly decreased. Moreover, the 16S rRNA sequencing analysis revealed that the changes in the gut microbiota caused by constipation were restored by d-tagatose treatment. In conclusion, this study indicated that the administration of d-tagatose as a dietary supplement can effectively prevent and relieve constipation in Kunming mice, and it is a promising prebiotic candidate with constipation-relieving properties.