Antiviral agent fTDP stimulates the SA signaling pathway and enhances tobacco defense against tobacco mosaic virus
Antiviral agent fTDP stimulates the SA signaling pathway and enhances tobacco defense against tobacco mosaic virus
复制标题
抗病毒剂 fTDP 刺激 SA 信号通路并增强烟草对烟草花叶病毒的防御
DOI：
10.1016/j.pestbp.2021.105002
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发表时间：
2021-12-23
影响因子：
4.7
通讯作者：
Wu, Lan
中科院分区：
文献类型：
--
作者：
Han, Xiaoxiao;Zhang, Yahong;Wu, Lan
金针菇TDP是抗烟草花叶病毒(TMV)的抗病毒源。然而，抗性机制尚未阐明。在本研究中，通过原核融合表达系统获得的融合蛋白fTDP对烟草花叶病毒具有明显的保护作用，并显著抑制了烟草花叶病毒的繁殖。转录组学和蛋白质组学分析表明，fTDP可能与受体相互作用，激活丝裂原活化蛋白激酶(MAPK)途径和NB-ARC，增加活性氧(ROS)和水杨酸(SA)含量，从而促进超敏反应(HR)和系统获得性抗性(SAR)。SARS引起过氧化氢酶(CAT)、病程相关蛋白1(PR1)、苯丙氨酸解氨酶(PAL)以及查尔酮-二氢黄酮异构酶(CHI)和细胞色素P450等参与病原菌防御的蛋白表达增加。综上所述，fTDP诱导的SAR具有保护烟草免受TMV侵染和缓解症状的作用。本研究为TDP蛋白的应用提供了理论依据，TDP蛋白可能是一种潜在的生物农药。
TEER-decreasing protein (TDP) from Flammulina velutipes was antiviral resource against tobacco mosaic virus (TMV). However, the resistance mechanisms have not been clarified. In this study, the fTDP (fusion teerdecreasing protein), obtained by prokaryotic fusion expression system, exhibited obvious protective efficacy against TMV and significantly suppressed the reproduction of TMV in tobacco. Transcriptomics and proteomics analysis showed that fTDP may interact with a receptor, activate the mitogen-activated protein kinase (MAPK) pathway and NB-ARC and increase the content of reactive oxygen species (ROS) and salicylic acid (SA), which promoted the hypersensitive response (HR) and system acquired resistance (SAR). SAR caused increased expression of catalase (CAT), pathogenesis-related protein 1 (PR1), phenylalanine ammonia lyase (PAL) and other proteins involved in pathogen defense, such as chalcone-dihydroflavone isomerase (CHI) and cytochrome P450. In conclusion, SAR was induced by fTDP to protect tobacco from TMV infection and alleviate the symptoms caused by the virus. The study provided a theoretical basis for the application of the TDP protein, which may represent a potential biopesticide.