In vivo metabolic activity of a putative circadian oscillator, the rat suprachiasmatic nucleus
In vivo metabolic activity of a putative circadian oscillator, the rat suprachiasmatic nucleus
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假定的昼夜节律振荡器（大鼠视交叉上核）的体内代谢活动
DOI：
10.1002/cne.901890109
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发表时间：
1980
影响因子：
2.5
通讯作者：
C. B. Smith
中科院分区：
文献类型：
--
作者：
W. Schwartz;L. Davidsen;C. B. Smith
视交叉上核（SCN）被认为是哺乳动物内源性昼夜节律振荡器的一个部位，因为该核的损伤会破坏广泛的明显的昼夜节律。为了证明完整（未损伤）动物SCN本身的可直接测量性质受环境光的影响并表现出昼夜节律性，我们使用放射自显影2-脱氧-D-[14 C]葡萄糖方法来测定各种光照条件下大鼠SCN的葡萄糖利用率。我们的实验表明SCN在昼夜节律组织中的重要作用，我们相信脱氧葡萄糖方法将被证明是一种有用的工具，用于更好地理解生物钟的功能和机制。
The suprachiasmatic nucleus (SCN) has been proposed as a site for an endogenous circadian oscillator in mammals, since lesions of the nucleus abolish a wide spectrum of overt circadian rhythms. To demonstrate that a directly measurable property of the SCN itself in intact (unlesioned) animals is affected by environmental light and exhibits circadian rhythmicity, we used the autoradiographic 2‐deoxy‐D‐[14C]glucose method to determine glucose utilization of rat SCN under a variety of lighting conditions. Our experiments indicate an important role for the SCN in circadian rhythm organization, and we believe the deoxyglucose method will prove useful as a tool for better understanding the functions and mechanisms of circadian clocks.