Dynamic de novo adipose tissue development during metamorphosis in Drosophila melanogaster
Dynamic de novo adipose tissue development during metamorphosis in Drosophila melanogaster
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DOI：
10.1101/2022.03.29.486213
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发表时间：
2022-03
期刊：
影响因子：
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通讯作者：
T. Tsuyama;Hanae Komai;Yusaku Hayashi;K. Shimono;T. Uemura
中科院分区：
文献类型：
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作者：
T. Tsuyama;Hanae Komai;Yusaku Hayashi;K. Shimono;T. Uemura
脂肪组织是控制无脊椎动物和脊椎动物全身代谢的中心器官。在这里，我们研究了果蝇成人型脂肪体(AFB)发育的细胞机制。我们已经建立了遗传工具，允许可视化和遗传操作的AFB谱系中的细胞从变态的早期。我们鉴定了产生黄曲霉毒素的前体细胞，并描述了黄曲霉毒素形成的动态细胞机制。这些前体细胞显示出极化的细胞形状和定向运动，从胸腔向外迁移，随后扩散到腹部和头部。迁移期过后，这些细胞相互粘连，组装成具有片状结构的AFB。在大规模迁移过程中和迁移后，细胞持续增殖，以获得适当的脂肪组织质量。薄片形成后的同型细胞融合有助于AFB内多核细胞的建立。我们还研究了候选基因的功能，我们的结果表明，rac1、蜕皮激素信号转导和转录因子Serpent支持成人脂肪体的器官发生。果蝇成体脂肪体前体细胞在变态过程中通过定向迁移、持续细胞增殖和同型细胞融合形成成体脂肪组织。
Adipose tissue is a central organ for controlling systemic metabolism both in invertebrates and vertebrates. Here, we have investigated the cellular mechanisms of the adult-type fat body (AFB) development in Drosophila. We have established genetic tools that allow visualization and genetic manipulations of cells in the AFB lineage from early in metamorphosis. We identified precursor cells that give rise to the AFB and delineated dynamic cellular mechanisms underlying AFB formation. These precursor cells displayed polarized cell shapes and oriented motility, with emigration from the thorax and subsequent dispersal to the abdomen and head. After the migration period, these cells adhered to each other, assembling into the AFB with a sheet-like architecture. Continuous cell proliferation occurred during and after the large-scale migration to achieve appropriate fat tissue mass. Homotypic cell fusion after the sheet formation contributed to the establishment of multinucleated cells in the AFB. We also examined candidate gene functions, and our results argue that Rac1, ecdysone signaling, and the transcription factor Serpent support adult fat body organogenesis. Brief Summary Statement Drosophila adult fat body precursor cells form adult adipose tissue during metamorphosis by directional migration, continuous cell proliferation, and homotypic cell fusion.