A PCR-based approach for isolating pathogen resistance genes from potato with potential for wide application in plants
A PCR-based approach for isolating pathogen resistance genes from potato with potential for wide application in plants
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DOI：
10.1038/ng1296-421
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发表时间：
1996-12-01
期刊：
影响因子：
30.8
通讯作者：
Gebhardt, C
中科院分区：
文献类型：
--
作者：
Leister, D;Ballvora, A;Gebhardt, C
植物的抗病基因可以用来改造抗病作物。从烟草和拟南芥抗性基因之间保守的序列基序中设计了寡核苷酸，并将其作为马铃薯DNA的聚合酶链式反应引物。扩增产物与已知抗病基因同源，与马铃薯抗线虫基因Gro1和马铃薯晚疫病菌抗病基因R7无重组连锁。马铃薯基因片段的MAP位置也与相关番茄和烟草基因组的抗性基因座相关联。我们的结果表明，基于共同的序列基序和PCR方法，可以分离出对线虫、真菌、病毒和细菌有效的植物抗性基因。
Plant genes for pathogen resistance can be used to engineer disease resistant crops. Oligonucleotides were designed from sequence motifs conserved between resistance genes of tobacco and Arabidopsis thaliana and used as PCR primers in potato DNA. Amplification products were obtained that were homologous to known resistance genes and linked without recombination with the nematode resistance locus Gro1 and the Phytophthora infestans resistance locus R7 of potato. Map positions of PCR-derived potato gene fragments were also correlated with resistance loci of the related tomato and tobacco genomes. Our results indicate that plant resistance genes that are effective against nematodes, fungi,viruses and bacteria may be isolated based on common sequence motifs and PCR methodology.