PROBING MITOCHONDRIA IN LIVING CELLS WITH RHODAMINE-123
PROBING MITOCHONDRIA IN LIVING CELLS WITH RHODAMINE-123
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DOI：
10.1101/sqb.1982.046.01.018
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
LAMPIDIS, TJ
中科院分区：
文献类型：
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作者：
CHEN, LB;SUMMERHAYES, IC;LAMPIDIS, TJ
自1850年Kolliker对颗粒（无疑是线粒体）的最初描述，以及随后Altmann（1890）对线粒体的研究以来，为了阐明这种复杂细胞器的结构和功能，已经进行了大量的工作。对线粒体的广泛的生化研究已经证明它们是有氧细胞的动力源，提供细胞生存和生长所必需的ATP （Lehninger 1964; Racker 1976）。尽管对分离的线粒体和固定细胞中的线粒体已经做了大量的工作，但对线粒体在其原生环境下的关注却少得多：活细胞。在最早对活线粒体进行的详细研究之一（Lewis and Lewis 1914）中，Lewis夫妇对培养的鸡胚胎细胞中线粒体的可塑性、运动性和形态异质性进行了有趣的描述。线粒体作为一种极具活力的细胞器的报道引发了对活线粒体特征的进一步尝试，并提出了线粒体在细胞质中的运动、线粒体的分裂和融合、大小变化、形状变化、方向和分布以及这些方面与细胞周期变化之间可能存在的相关性的各种问题。线粒体以大小不一的颗粒、棒状、线状、环状或分枝网络的形式存在，其形状和大小是无限的。铁道部,
Since the initial description of granules, which undoubtedly were mitochondria, by Kolliker in 1850 and since the subsequent studies of mitochondria by Altmann (1890), much work has been carried out in order to elucidate both the structure and function of this complex organelle. Extensive biochemical studies of the mitochondria have proved them to be the powerhouses of the aerobic cells, providing the ATP necessary for cell survival and growth (Lehninger 1964; Racker 1976).Although a vast amount of work has been done with isolated mitochondria and mitochondria in fixed cells, much less attention has been paid to the mitochondrion in its native context: the live cell. In one of the earliest detailed studies of live mitochondria (Lewis and Lewis 1914), the Lewises presented an intriguing description of mitochondrial plasticity, motility, and morphological heterogeneity in cultured chick embryo cells. This report of the mitochondrion as an extremely dynamic organelle provoked further attempts to characterize the live mitochondrion and raised various questions concerning mitochondrial movement in the cytoplasm, mitochondrial fission and fusion, size change, shape change, orientation and distribution, and a possible correlation between these aspects and changes in cell cycle. Found as large or small granules, rods, threads, loops, rings, or branched networks, mitochondria assume an infinite number of shapes and sizes. Mor-