GPR54 peptide agonists stimulate insulin secretion from murine, porcine and human islets
GPR54 peptide agonists stimulate insulin secretion from murine, porcine and human islets
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DOI：
10.4161/isl.18261
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发表时间：
2012-01-01
期刊：
影响因子：
2.2
通讯作者：
Persaud, Shanta J.
中科院分区：
文献类型：
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作者：
Bowe, James E.;Foot, Victoria L.;Persaud, Shanta J.
本研究旨在确定kisspeptin的10和13个氨基酸形式对哺乳动物胰岛动态胰岛素分泌的影响，因为从已发表的数据中尚不清楚较短的肽是否具有刺激作用，而较长的肽是否抑制胰岛素释放。用kisspeptin-10或kisspeptin-13灌注人、猪、大鼠和小鼠离体胰岛，在20 mM葡萄糖存在下，用放射免疫法测定胰岛素分泌。这两种多肽刺激了所有实验物种的胰岛中葡萄糖刺激的胰岛素分泌的快速、可逆的增强。这些数据表明，kisspeptin-10和kisspeptin-13都是kisspeptin-10的三个氨基酸延伸，直接作用于不同物种的胰岛β细胞以增强胰岛素分泌，并提示早期研究报道的抑制作用可能反映了实验方案的差异。
This study was designed to determine the effects of 10 and 13 amino acid forms of kisspeptin on dynamic insulin secretion from mammalian islets since it is not clear from published data whether the shorter peptide is stimulatory while the longer peptide inhibits insulin release. Insulin secretion was measured by radioimmunoassay following perifusion of human, pig, rat and mouse isolated islets with kisspeptin-10 or kisspeptin-13 in the presence of 20 mM glucose. Both peptides stimulated rapid, reversible potentiation of glucose-stimulated insulin secretion from islets of all species tested. These data indicate that both kisspeptin-10 and kisspeptin-13, which is an extension of kisspeptin-10 by three amino acids, act directly at islet beta-cells of various species to potentiate insulin secretion, and suggest that inhibitory effects reported in earlier studies may reflect differences in experimental protocols.